ArticleScientific reports2016
Polarization-dependent fluorescence correlation spectroscopy for studying structural properties of proteins in living cell.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 24 citations in OpenAlex.
- Exploring Rotational Diffusion with Plasmonic Coupling.ACS photonics · 2024Article
- Optical Polarization-Based Measurement Methods for Characterization of Self-Assembled Peptides' and Amino Acids' Micro- and Nanostructures.Molecules (Basel, Switzerland) · 2022Review
- Unraveling the hidden temporal range of fast βCommunications biology · 2022Article
- Empirical Bayes method using surrounding pixel information for number and brightness analysis.Biophysical journal · 2021Article
- Quantitative evaluation of macromolecular crowding environment based on translational and rotational diffusion using polarization dependent fluorescence correlation spectroscopy.Scientific reports · 2021Article
- POLArIS, a versatile probe for molecular orientation, revealed actin filaments associated with microtubule asters in early embryos.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Semiconductor Nanocrystals for Biological Imaging and Fluorescence Spectroscopy.Advances in experimental medicine and biology · 2021Article
- Directionality of light absorption and emission in representative fluorescent proteins.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Nanoscale Viscosity of Cytoplasm Is Conserved in Human Cell Lines.The journal of physical chemistry letters · 2020Article
- Mouse Heterochromatin Adopts Digital Compaction States without Showing Hallmarks of HP1-Driven Liquid-Liquid Phase Separation.Molecular cell · 2020Article
- Multipoint fluorescence correlation spectroscopy using spatial light modulator.Biomedical optics express · 2018Article
- State-of-the-Art Fluorescence Fluctuation-Based Spectroscopic Techniques for the Study of Protein Aggregation.International journal of molecular sciences · 2018Review
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rotational diffusion measurement is predicted as an important method in cell biology because the rotational properties directly reflect molecular interactions and environment in the cell. To prove this concept, polarization-dependent fluorescence correlation spectroscopy (pol-FCS) measurements of purified fluorescent proteins were conducted in viscous solution. With the comparison between the translational and rotational diffusion coefficients obtained from pol-FCS measurements, the hydrodynamic radius of an enhanced green fluorescent protein (EGFP) was estimated as a control measurement. The orientation of oligomer EGFP in living cells was also estimated by pol-FCS and compared with Monte Carlo simulations. The results of this pol-FCS experiment indicate that this method allows an estimation of the molecular orientation using the characteristics of rotational diffusion. Further, it can be applied to analyze the degree of molecular orientation and multimerization or detection of tiny aggregation of aggregate-prone proteins.
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