ArticleAmerican journal of physiology. Renal physiology2016
Phosphatase inhibition increases AQP2 accumulation in the rat IMCD apical plasma membrane.
Article in American journal of physiology. Renal physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 28 citations in OpenAlex.
- Age-Related Adaptations in Renal Tubular Function in Female Rats.Acta physiologica (Oxford, England) · 2026Article
- Exploring Aquaporins in Human Studies: Mechanisms and Therapeutic Potential in Critical Illness.Life (Basel, Switzerland) · 2024Review
- Activation of TGR5 Increases Urine Concentration by Inducing AQP2 and AQP3 Expression in Renal Medullary Collecting Ducts.Kidney diseases (Basel, Switzerland) · 2024Article
- Aldosterone Contributes to Vasopressin Escape through Changes in Water and Urea Transport.Biomedicines · 2023Article
- Phosphatases Decrease Water and Urea Permeability in Rat Inner Medullary Collecting Ducts.International journal of molecular sciences · 2023Article
- Aquaporins in Urinary System.Advances in experimental medicine and biology · 2023Article
- Identification of AGXT2, SHMT1, and ACO2 as important biomarkers of acute kidney injury by WGCNA.PloS one · 2023Article
- Effects of a Chinese herbal extract on the intestinal tract and aquaporin in Adriamycin-induced nephropathy.Bioengineered · 2022Article
- Renal ATCirculation research · 2022Article
- Role of protein phosphatase 2A in kidney disease (Review).Experimental and therapeutic medicine · 2021Review
- Sex-specific adaptations to high-salt diet preserve electrolyte homeostasis with distinct sodium transporter profiles.American journal of physiology. Cell physiology · 2021Article
- Olive Leaf Extract (OLE) impaired vasopressin-induced aquaporin-2 trafficking through the activation of the calcium-sensing receptor.Scientific reports · 2021Article
- Aldosterone Decreases Vasopressin-Stimulated Water Reabsorption in Rat Inner Medullary Collecting Ducts.Cells · 2020Article
- Targeting the Trafficking of Kidney Water Channels for Therapeutic Benefit.Annual review of pharmacology and toxicology · 2020Review
- Involvement of PDZ-SAP97 interactions in regulating AQP2 translocation in response to vasopressin in LLC-PKAmerican journal of physiology. Renal physiology · 2019Article
- Urinary extracellular vesicular release of aquaporins in patients with renal transplantation.BMC nephrology · 2019Article
- Review
- Aquaporins in Renal Diseases.International journal of molecular sciences · 2019Review
- Protein phosphatase 2C is responsible for VP-induced dephosphorylation of AQP2 serine 261.American journal of physiology. Renal physiology · 2017Article
- Serine/threonine phosphatases and aquaporin-2 regulation in renal collecting duct.American journal of physiology. Renal physiology · 2017Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Vasopressin triggers the phosphorylation and apical plasma membrane accumulation of aquaporin 2 (AQP2), and it plays an essential role in urine concentration. Vasopressin, acting through protein kinase A, phosphorylates AQP2. However, the phosphorylation state of AQP2 could also be affected by the action of protein phosphatases (PPs). Rat inner medullas (IM) were incubated with calyculin (PP1 and PP2A inhibitor, 50 nM) or tacrolimus (PP2B inhibitor, 100 nM). Calyculin did not affect total AQP2 protein abundance (by Western blot) but did significantly increase the abundances of pS256-AQP2 and pS264-AQP2. It did not change pS261-AQP2 or pS269-AQP2. Calyculin significantly enhanced the membrane accumulation (by biotinylation) of total AQP2, pS256-AQP2, and pS264-AQP2. Likewise, immunohistochemistry showed an increase in the apical plasma membrane association of pS256-AQP2 and pS264-AQP2 in calyculin-treated rat IM. Tacrolimus also did not change total AQP2 abundance but significantly increased the abundances of pS261-AQP2 and pS264-AQP2. In contrast to calyculin, tacrolimus did not change the amount of total AQP2 in the plasma membrane (by biotinylation and immunohistochemistry). Tacrolimus did increase the expression of pS264-AQP2 in the apical plasma membrane (by immunohistochemistry). In conclusion, PP1/PP2A regulates the phosphorylation and apical plasma membrane accumulation of AQP2 differently than PP2B. Serine-264 of AQP2 is a phosphorylation site that is regulated by both PP1/PP2A and PP2B. This dual regulatory pathway may suggest a previously unappreciated role for multiple phosphatases in the regulation of urine concentration.
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