Evidence map›Paper›PMID 27488614›Full record

ArticleJournal of ovarian research2016

An ovarian bioreactor for in vitro culture of the whole bovine ovary: a preliminary report.

Matthew R Zanotelli, Joseph D Henningsen, Patrick M Hopkins, Aaron P Dederich, Tessa Herman, Tracy J Puccinelli, Sana M Salih

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of ovarian research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact, top 92% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 10 citations in OpenAlex.

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  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Matthew R ZanotelliDepartment of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA.
Joseph D HenningsenDepartment of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA.
Patrick M HopkinsDepartment of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA.
Aaron P DederichDepartment of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA.
Tessa HermanDepartment of Obstetrics and Gynecology, West Virginia University, Morgantown, WV, 26506, USA.
Tracy J PuccinelliDepartment of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA.
Sana M SalihDepartment of Obstetrics and Gynecology, West Virginia University, Morgantown, WV, 26506, USA. sana.salih@hsc.wvu.edu.
University of Wisconsin–Madison · US

Funding

UW COMPREHENSIVE CANCER CENTER SUPPORTP30CA014520 · NCI · UNIVERSITY OF WISCONSIN-MADISON · PI AMY TRENTHAM-DIETZ · 1985 to 2026
$142.6M
Institutional Clinical and Translational Science AwardUL1TR000427 · NCATS · UNIVERSITY OF WISCONSIN-MADISON · PI DREZNER, MARC KENNETH · 2012 to 2016
$32.2M
NCATS NIH HHS UL1 TR000427NCI NIH HHS P30 CA014520
6 · The paper itself

Abstract

backgroundImproved cancer therapeutics and enhanced cancer survivorship have emphasized the severe long-term side effects of chemotherapy. Specifically, studies have linked many chemotherapy agents with primary ovarian insufficiency, although an exact insult model has not yet been determined. To investigate and ultimately solve this problem, a novel device for extended study of mammalian ovaries in vitro was developed.

methodsA bioreactor was fabricated for bovine ovarian culture that provides intravascular delivery of media to the ovary through isolation and cannulation of a main ovarian artery branch. Whole ovaries were cultured in vitro using three methods: (1) continuously supplied fresh culture media, (2) recirculated culture media, or (3) continuously supplied fresh culture media supplemented with 500 nM doxorubicin for 24 or 48 h. TUNEL assay was used to assess apoptotic cell percentages in the three groups as compared to uncultured baseline ovaries.

resultsThe ovary culture method was shown to maintain cell viability by effectively delivering nutrient-enriched pH-balanced media at a constant flow rate. Lower apoptosis observed in ovaries cultured in continuously supplied fresh culture media illustrates that this culture device and method are the first to sustain whole bovine ovary viability for 48 h. Meanwhile, the increase in the percentage of cell apoptosis with doxorubicin treatment indicates that the device can provide an alternative model for testing chemotherapy and chemoprotection treatments to prevent primary ovarian insufficiency in cancer patients.

conclusionsAn ovarian bioreactor with consistent culture media flow through an ovarian vasculature-assisted approach maintains short-term whole bovine ovary viability.

Indexed as

AnimalsBioreactorsCattleCell SurvivalCulture MediaDoxorubicinFemaleHumansOrgan Culture TechniquesOvaryCulture MediaDoxorubicinApoptosisBioreactorBovine ovaryChemotherapyDoxorubicinGranulosa cellInfertilityOvarian insufficiencyOvaryOvary culture

Identifiers

PMID27488614
PMCPMC4973044
OpenAlexW2477049290

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.