ArticleStem cell research & therapy2016
Significant improvement of direct reprogramming efficacy of fibroblasts into progenitor endothelial cells by ETV2 and hypoxia.
Article in Stem cell research & therapy, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 27 citations in OpenAlex.
- Revisiting Pluripotency Acquisition: A Unified Framework for Reprogramming Strategies.Development, growth & differentiation · 2026Review
- Review
- SOX17/ETV2 improves the direct reprogramming of adult fibroblasts to endothelial cells.Cell reports methods · 2024Article
- ETV2/ER71, the key factor leading the paths to vascular regeneration and angiogenic reprogramming.Stem cell research & therapy · 2023Review
- Updated Perspectives on Direct Vascular Cellular Reprogramming and Their Potential Applications in Tissue Engineered Vascular Grafts.Journal of functional biomaterials · 2022Review
- Article
- Slight up-regulation of Kir2.1 channel promotes endothelial progenitor cells to transdifferentiate into a pericyte phenotype by Akt/mTOR/Snail pathway.Journal of cellular and molecular medicine · 2021Article
- Direct cell-fate conversion of somatic cells: Toward regenerative medicine and industries.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2020Review
- CRISPR-Knockout Screen Identifies Dmap1 as a Regulator of Chemically Induced Reprogramming and Differentiation of Cardiac Progenitors.Stem cells (Dayton, Ohio) · 2019Article
- Direct cell reprogramming for tissue engineering and regenerative medicine.Journal of biological engineering · 2019Review
- ETV2/ER71 Transcription Factor as a Therapeutic Vehicle for Cardiovascular Disease.Theranostics · 2019Review
- Bioactive Molecules for Skin Repair and Regeneration: Progress and Perspectives.Stem cells international · 2019Review
- Conversion of human adipose-derived stem cells into functional and expandable endothelial-like cells for cell-based therapies.Stem cell research & therapy · 2018Article
- Cooperation between ETS variant 2 and Jumonji domain‑containing 2 histone demethylases.Molecular medicine reports · 2018Article
- Regenerating the Cardiovascular System Through Cell Reprogramming; Current Approaches and a Look Into the Future.Frontiers in cardiovascular medicine · 2018Review
- Evaluation of human dermal fibroblasts directly reprogrammed to adipocyte-like cells as a metabolic disease model.Disease models & mechanisms · 2017Article
- Extracellular vesicles of ETV2 transfected fibroblasts stimulate endothelial cells and improve neovascularization in a murine model of hindlimb ischemia.Cytotechnology · 2017Article
- Etv2 as an essential regulator of mesodermal lineage development.Cardiovascular research · 2017Review
- ETV-2 activated proliferation of endothelial cells and attenuated acute hindlimb ischemia in mice.In vitro cellular & developmental biology. Animal · 2017Article
- Production of endothelial progenitor cells from skin fibroblasts by direct reprogramming for clinical usages.In vitro cellular & developmental biology. Animal · 2017Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundEndothelial progenitor cell (EPC) transplantation is a promising therapy for ischemic diseases such as ischemic myocardial infarction and hindlimb ischemia. However, limitation of EPC sources remains a major obstacle. Direct reprogramming has become a powerful tool to produce EPCs from fibroblasts. Some recent efforts successfully directly reprogrammed human fibroblasts into functional EPCs; however, the procedure efficacy was low. This study therefore aimed to improve the efficacy of direct reprogramming of human fibroblasts to functional EPCs.
methodsHuman fibroblasts isolated from foreskin were directly reprogrammed into EPCs by viral ETV2 transduction. Reprogramming efficacy was improved by culturing transduced fibroblasts in hypoxia conditions (5 % oxygen). Phenotype analyses confirmed that single-factor ETV2 transduction successfully reprogrammed dermal fibroblasts into functional EPCs.
resultsHypoxia treatment during the reprogramming procedure increased the efficacy of reprogramming from 1.21 ± 0.61 % in normoxia conditions to 7.52 ± 2.31 % in hypoxia conditions. Induced EPCs in hypoxia conditions exhibited functional EPC phenotypes similar to those in normoxia conditions, such as expression of CD31 and VEGFR2, and expressed endothelial gene profiles similar to human umbilical vascular endothelial cells. These cells also formed capillary-like networks in vitro.
conclusionOur study demonstrates a new simple method to increase the reprogramming efficacy of human fibroblasts to EPCs using ETV2 and hypoxia.
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