ArticleThe American journal of clinical nutrition2016
Biomarkers of browning of white adipose tissue and their regulation during exercise- and diet-induced weight loss.
Article in The American journal of clinical nutrition, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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37 citing papers in PubMed, 67 citations in OpenAlex.
- Combined training increases thermogenic fat activity in patients with overweight and type 2 diabetes.International journal of obesity (2005) · 2022Trial
- Exercise and Fat: A Primer.Sports medicine - open · 2026Review
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- Obesity and Exercise: New Insights and Perspectives.Endocrine reviews · 2025Review
- Housing temperature influences exercise-induced glucose regulation and expression of exerkines in mice.Experimental physiology · 2025Article
- Sex-specific skeletal muscle gene expression responses to exercise reveal novel direct mediators of insulin sensitivity change.NAR molecular medicine · 2025Article
- Can Exercise-Mediated Adipose Browning Provide an Alternative Explanation for the Obesity Paradox?International journal of molecular sciences · 2025Review
- Brown and Beige Adipose Tissue: One or Different Targets for Treatment of Obesity and Obesity-Related Metabolic Disorders?International journal of molecular sciences · 2024Review
- The Different Shades of Thermogenic Adipose Tissue.Current obesity reports · 2024Review
- The characterization of metabolic changes in adipose tissues and muscles due to different exercise intensities by Dixon in healthy young men.European journal of radiology · 2024Article
- Article
- Transcriptomics for Clinical and Experimental Biology Research: Hang on a Seq.Advanced genetics (Hoboken, N.J.) · 2023Review
- A human-based multi-gene signature enables quantitative drug repurposing for metabolic disease.eLife · 2022Article
- The confounding effects of sub-thermoneutral housing temperatures on aerobic exercise-induced adaptations in mouse subcutaneous white adipose tissue.Biology letters · 2021Article
- Exercise Training Promotes Sex-Specific Adaptations in Mouse Inguinal White Adipose Tissue.Diabetes · 2021Article
- Human white-fat thermogenesis: Experimental and meta-analytic findings.Temperature (Austin, Tex.) · 2020Article
- Impacts of rat hindlimb Fndc5/irisin overexpression on muscle and adipose tissue metabolism.American journal of physiology. Endocrinology and metabolism · 2020Article
- Brown Adipose Crosstalk in Tissue Plasticity and Human Metabolism.Endocrine reviews · 2020Review
- Exercise-Induced Adaptations to Adipose Tissue Thermogenesis.Frontiers in endocrinology · 2020Review
- Effects of Royal Jelly and Tocotrienol Rich Fraction in obesity treatment of calorie-restricted obese rats: a focus on white fat browning properties and thermogenic capacity.Nutrition & metabolism · 2020Article
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10 authors at 6 institutions in 3 countries.
Funding
Abstract
backgroundA hypothesis exists whereby an exercise- or dietary-induced negative energy balance reduces human subcutaneous white adipose tissue (scWAT) mass through the formation of brown-like adipocyte (brite) cells. However, the validity of biomarkers of brite formation has not been robustly evaluated in humans, and clinical data that link brite formation and weight loss are sparse.
objectivesWe used rosiglitazone and primary adipocytes to stringently evaluate a set of biomarkers for brite formation and determined whether the expression of biomarker genes in scWAT could explain the change in body composition in response to exercise training combined with calorie restriction in obese and overweight women (n = 79).
designGene expression was derived from exon DNA microarrays and preadipocytes from obesity-resistant and -sensitive mice treated with rosiglitazone to generate candidate brite biomarkers from a microarray. These biomarkers were evaluated against data derived from scWAT RNA from obese and overweight women before and after supervised exercise 5 d/wk for 16 wk combined with modest calorie restriction (∼0.84 MJ/d).
resultsForty percent of commonly used brite gene biomarkers exhibited an exon or strain-specific regulation. No biomarkers were positively related to weight loss in human scWAT. Greater weight loss was significantly associated with less uncoupling protein 1 expression (P = 0.006, R(2) = 0.09). In a follow-up global analysis, there were 161 genes that covaried with weight loss that were linked to greater CCAAT/enhancer binding protein α activity (z = 2.0, P = 6.6 × 10(-7)), liver X receptor α/β agonism (z = 2.1, P = 2.8 × 10(-7)), and inhibition of leptin-like signaling (z = -2.6, P = 3.9 × 10(-5)).
conclusionWe identify a subset of robust RNA biomarkers for brite formation and show that calorie-restriction-mediated weight loss in women dynamically remodels scWAT to take on a more-white rather than a more-brown adipocyte phenotype.
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