ArticleBiology of reproduction2016
Reactive Oxygen Stimulation of Interleukin-6 Release in the Human Trophoblast Cell Line HTR-8/SVneo by the Trichlorethylene Metabolite S-(1,2-Dichloro)-l-Cysteine.
Article in Biology of reproduction, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 24 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it, 34 citations in OpenAlex.
- Environmental contaminants and preeclampsia: a systematic literature review.Journal of toxicology and environmental health. Part B, Critical reviews · 2018Pooled it
- Trichloroethylene: An Update on an Environmental Contaminant with Multiple Health Effects.Annual review of pharmacology and toxicology · 2025Review
- Article
- Trichloroethylene MetaboliteChemical research in toxicology · 2023Article
- Apoptotic responses stimulated by the trichloroethylene metabolite S-(1,2-dichlorovinyl)-L-cysteine depend on cell differentiation state in BeWo human trophoblast cells.Toxicology in vitro : an international journal published in association with BIBRA · 2023Article
- Oxidative stress on vessels at the maternal-fetal interface for female reproductive system disorders: Update.Frontiers in endocrinology · 2023Review
- The trichloroethylene metabolite S-(1,2-dichlorovinyl)-l-cysteine inhibits lipopolysaccharide-induced inflammation transcriptomic pathways and cytokine secretion in a macrophage cell model.Toxicology in vitro : an international journal published in association with BIBRA · 2022Article
- Toxicity assessments of selected trichloroethylene and perchloroethylene metabolites in three in vitro human placental models.Reproductive toxicology (Elmsford, N.Y.) · 2022Article
- Trichloroethylene modifies energy metabolites in the amniotic fluid of Wistar rats.Reproductive toxicology (Elmsford, N.Y.) · 2022Article
- PM10 Alters Trophoblast Cell Function and Modulates miR-125b-5p Expression.BioMed research international · 2022Article
- Preeclampsia and Neurodevelopmental Outcomes: Potential Pathogenic Roles for Inflammation and Oxidative Stress?Molecular neurobiology · 2021Review
- Transcriptional profiling of the response to the trichloroethylene metabolite S-(1,2-dichlorovinyl)-L-cysteine revealed activation of the eIF2α/ATF4 integrated stress response in two in vitro placental models.Archives of toxicology · 2021Article
- N-Acetyl-L-cysteine and aminooxyacetic acid differentially modulate trichloroethylene reproductive toxicity via metabolism in Wistar rats.Archives of toxicology · 2021Article
- Preeclampsia: From Cellular Wellness to Inappropriate Cell Death, and the Roles of Nutrition.Frontiers in cell and developmental biology · 2021Review
- Tert-Butyl Hydroperoxide Stimulated Apoptosis Independent of Prostaglandin EReproductive sciences (Thousand Oaks, Calif.) · 2020Article
- Exposure to Trichloroethylene MetaboliteChemical research in toxicology · 2020Article
- Placenta as a target of trichloroethylene toxicity.Environmental science. Processes & impacts · 2020Article
- The trichloroethylene metabolite S-(1,2-dichlorovinyl)-L-cysteine induces progressive mitochondrial dysfunction in HTR-8/SVneo trophoblasts.Toxicology · 2019Article
- Ozone Exposure During Implantation Increases Serum Bioactivity in HTR-8/SVneo Trophoblasts.Toxicological sciences : an official journal of the Society of Toxicology · 2019Article
- New Insights into the Process of Placentation and the Role of Oxidative Uterine Microenvironment.Oxidative medicine and cellular longevity · 2019Review
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Trichloroethylene (TCE) is a common environmental pollutant associated with adverse reproductive outcomes in humans. TCE intoxication occurs primarily through its biotransformation to bioactive metabolites, including S-(1,2-dichlorovinyl)-l-cysteine (DCVC). TCE induces oxidative stress and inflammation in the liver and kidney. Although the placenta is capable of xenobiotic metabolism and oxidative stress and inflammation in placenta have been associated with adverse pregnancy outcomes, TCE toxicity in the placenta remains poorly understood. We determined the effects of DCVC by using the human extravillous trophoblast cell line HTR-8/SVneo. Exposure to 10 and 20 μM DCVC for 10 h increased reactive oxygen species (ROS) as measured by carboxydichlorofluorescein fluorescence. Moreover, 10 and 20 μM DCVC increased mRNA expression and release of interleukin-6 (IL-6) after 24-h exposure, and these responses were inhibited by the cysteine conjugate beta-lyase inhibitor aminooxyacetic acid and by treatments with antioxidants (alpha-tocopherol and deferoxamine), suggesting that DCVC-stimulated IL-6 release in HTR-8/SVneo cells is dependent on beta-lyase metabolic activation and increased generation of ROS. HTR-8/SVneo cells exhibited decreased mitochondrial membrane potential at 5, 10, and 20 μM DCVC at 5, 10, and 24 h, showing that DCVC induces mitochondrial dysfunction in HTR-8/Svneo cells. The present study demonstrates that DCVC stimulated ROS generation in the human placental cell line HTR-8/SVneo and provides new evidence of mechanistic linkage between DCVC-stimulated ROS and increase in proinflammatory cytokine IL-6. Because abnormal activation of cytokines can disrupt trophoblast functions necessary for placental development and successful pregnancy, follow-up investigations relating these findings to physiologic outcomes are warranted.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.