ArticleThe Indian journal of medical research2016
Isolation & characterization of Brucella melitensis isolated from patients suspected for human brucellosis in India.
Article in The Indian journal of medical research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 33 citations in OpenAlex.
- A Systematic Review and Meta-Analysis of Epidemiology and Clinical Manifestations of Human Brucellosis in China.BioMed research international · 2018Pooled it
- One-pot, uracil-DNA-glycosylase-aided, and multi-indicator nanobiosensor detection platform for identifying Brucella melitensis from the Brucella spp.BMC microbiology · 2025Article
- Genomic insights intoMicrobiology spectrum · 2025Article
- Human Brucellosis in the Rural and Urban Population of Pakistan: Seroprevalence, Risk Factors, and Clinical Manifestations.Current microbiology · 2025Article
- Protocol for a randomized controlled trial on the impact of a health action process approach model-based mobile health intervention via WeChat on health behaviors of brucellosis patients.Frontiers in public health · 2025Article
- Molecular epidemiology of brucellosis in Asia: insights from genotyping analyses.Veterinary research communications · 2024Review
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- Seroprevalence of Brucellosis in Patients Having Complained of Joint Pain: A Case Control.Cureus · 2023Article
- Rapid, ultrasensitive, and highly specific identification ofFrontiers in microbiology · 2022Article
- Article
- Rare case of brucellosis misdiagnosed as prostate carcinoma with lumbar vertebra metastasis: A case report.World journal of clinical cases · 2021Article
- Effect of educational intervention on preventive behaviors of brucellosis among health volunteers in Rafsanjan city: Application of health belief model.Journal of education and health promotion · 2021Article
- Optimization and validation of a real-time polymerase chain reaction protocol for the diagnosis of human brucellosis.Folia microbiologica · 2020Article
- Article
- Laboratory Diagnosis of Human Brucellosis.Clinical microbiology reviews · 2019Review
- Isolation and identification of Brucella melitensis using bacteriological and molecular tools from aborted goats in the Afar region of north-eastern Ethiopia.BMC microbiology · 2019Article
- Caprine brucellosis: A historically neglected disease with significant impact on public health.PLoS neglected tropical diseases · 2017Review
- Evaluation of recombinant porin (rOmp2a) protein as a potential antigen candidate for serodiagnosis of Human Brucellosis.BMC infectious diseases · 2017Article
- The clinical features of 590 patients with brucellosis in Xinjiang, China with the emphasis on the treatment of complications.PLoS neglected tropical diseases · 2017Article
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8 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND &
objectivesBrucellosis is endemic in the southern part of India. A combination of biochemical, serological and molecular methods is required for identification and biotyping of Brucella. The present study describes the isolation and biochemical, molecular characterization of Brucella melitensis from patients suspected for human brucellosis.
methodsThe blood samples were collected from febrile patients suspected to have brucellosis. A total of 18 isolates were obtained from 102 blood samples subjected to culture. The characterization of these 18 isolates was done by growth on Brucella specific medium, biochemical reactions, CO2 requirement, H2S production, agglutination with A and M mono-specific antiserum, dye sensitivity to basic fuchsin and thionin. Further, molecular characterization of the isolates was done by amplification of B. melitensis species specific IS 711 repetitive DNA fragment and 16S (rRNA) sequence analysis. PCR-restriction fragment length polymorphism (RFLP) analysis of omp2 locus and IS711 gene was also done for molecular characterization.
resultsAll 102 suspected samples were subjected to bacteria isolation and of these, 18 isolates could be recovered on blood culture. The biochemical, PCR and PCR-RFLP and 16s rRNA sequencing revealed that all isolates were of B. melitensis and matched exactly with reference strain B. melitensis 16M. INTERPRETATION &
conclusionsThe present study showed an overall isolation rate of 17.64 per cent for B. melitensis. There is a need to establish facilities for isolation and characterization of Brucella species for effective clinical management of the disease among patients as well as surveillance and control of infection in domestic animals. Further studies are needed from different geographical areas of the country with different level of endemicity to plan and execute control strategies against human brucellosis.
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