ArticlePloS one2016
Identification of Differentially-Expressed Genes in Response to Mycosphaerella fijiensis in the Resistant Musa Accession 'Calcutta-4' Using Suppression Subtractive Hybridization.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
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Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it, 40 citations in OpenAlex.
- Genetic Improvement for Resistance to Black Sigatoka in Bananas: A Systematic Review.Frontiers in plant science · 2021Pooled it
- Expression of Genes Involved in Banana (Current issues in molecular biology · 2024Article
- Phytoparasitic Nematodes ofPlants (Basel, Switzerland) · 2024Review
- Transcriptome Profiling of the Resistance Response ofInternational journal of molecular sciences · 2022Article
- Gene Expression, Histology and Histochemistry in the Interaction betweenPlants (Basel, Switzerland) · 2022Article
- Genetic Characteristics and Metabolic Interactions betweenPlants (Basel, Switzerland) · 2022Review
- Improvements in the Resistance of the Banana Species to Fusarium Wilt: A Systematic Review of Methods and Perspectives.Journal of fungi (Basel, Switzerland) · 2021Review
- Earthworm species inZooKeys · 2021Article
- A polyketide synthase gene cluster required for pathogenicity of Pseudocercospora fijiensis on banana.PloS one · 2021Article
- Dataset of suppression subtractive hybridization libraries of banana-biostimulant-Data in brief · 2019Article
- Metabolite profiling characterises chemotypes of Musa diploids and triploids at juvenile and pre-flowering growth stages.Scientific reports · 2019Article
- A novel polyketide synthase gene cluster in the plant pathogenic fungus Pseudocercospora fijiensis.PloS one · 2019Article
- A polyketide synthase gene cluster associated with the sexual reproductive cycle of the banana pathogen, Pseudocercospora fijiensis.PloS one · 2019Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bananas and plantains are considered an important crop around the world. Banana production is affected by several constraints, of which Black Sigatoka Disease, caused by the fungus Mycosphaerella fijiensis, is considered one of the most important diseases in banana plantations. The banana accession 'Calcutta-4' has a natural resistance to Black Sigatoka; however, the fruit is not valuable for commercialization. Gene identification and expression studies in 'Calcutta-4' might reveal possible gene candidates for resistant to the disease and elucidate mechanisms for resistance. A subtracted cDNA library was generated from leaves after 6, 9 and 12 days inoculated with M. fijiensis conidia on greenhouse banana plants of the accession 'Calcutta-4'. Bioinformatic analysis revealed 99 good quality sequences. Blast2go analysis revealed that 31% of the sequences could not be categorized and, according to the Biological Process Category, 32 and 28 ESTs are related to general metabolic and cellular processes, respectively; while 10 ESTs response to stimulus. Seven sequences were redundant and one was similar to genes that may be involved in pathogen resistance including the putative disease resistance protein RGA1. Genes encoding zinc finger domains were identified and may play an important role in pathogen resistance by inducing the expression of downstream genes. Expression analysis of four selected genes was performed using RT-qPCR during the early stage of the disease development at 6, 9, 12 and 15 days post inoculation showing a peak of up regulation at 9 or 12 days post inoculation. Three of the four genes showed an up-regulation of expression in 'Calcutta-4' when compared to 'Williams' after inoculation with M. fijiensis, suggesting a fine regulation of specific gene candidates that may lead to a resistance response. The genes identified in early responses in a plant-pathogen interaction may be relevant for the resistance response of 'Calcutta-4' to Black Sigatoka. Genes with different functions may play a role in plant response to the disease. The present study suggests a fine up regulation of these genes that might be needed to perform an incompatible interaction. Further gene functional studies need to be performed to validate their use as candidate resistance genes in susceptible banana cultivars.
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