Evidence map›Paper›PMID 27487171›Full record

ArticleHuman vaccines & immunotherapeutics2016

Production of Plasmodium vivax enolase in Escherichia coli and its protective properties.

Chao Zhang, Yaping Gu, Jianxia Tang, Feng Lu, Yuanyuan Cao, Huayun Zhou, Guoding Zhu, Jun Cao, Qi Gao

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Human vaccines & immunotherapeutics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
2.4field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 17 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Chao Zhanga Key Laboratory on Technology for Disease Prevention and Control (Ministry of Health) , Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases , Wuxi , Jiangsu , China.
Yaping Gua Key Laboratory on Technology for Disease Prevention and Control (Ministry of Health) , Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases , Wuxi , Jiangsu , China.
Jianxia Tanga Key Laboratory on Technology for Disease Prevention and Control (Ministry of Health) , Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases , Wuxi , Jiangsu , China.
Feng Lua Key Laboratory on Technology for Disease Prevention and Control (Ministry of Health) , Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases , Wuxi , Jiangsu , China.
Yuanyuan Caoa Key Laboratory on Technology for Disease Prevention and Control (Ministry of Health) , Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases , Wuxi , Jiangsu , China.
Huayun Zhoua Key Laboratory on Technology for Disease Prevention and Control (Ministry of Health) , Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases , Wuxi , Jiangsu , China.
Guoding Zhua Key Laboratory on Technology for Disease Prevention and Control (Ministry of Health) , Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases , Wuxi , Jiangsu , China.
Jun Caoa Key Laboratory on Technology for Disease Prevention and Control (Ministry of Health) , Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases , Wuxi , Jiangsu , China.
Qi Gaoa Key Laboratory on Technology for Disease Prevention and Control (Ministry of Health) , Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases , Wuxi , Jiangsu , China.
Jiangsu Institute of Parasitic Diseases · CNJiangnan University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Plasmodium vivax predominates in South-East Asia and the American continent, causes significant morbidity and inflicts a huge socioeconomic burden. Sequencing completion of the Plasmodium vivax genome and transcriptome provides the chance to identify antigens. Enolase is the eighth enzyme in the glycolytic pathway, which, apart from its glycolytic function, also possess antigenic properties and is present on the cell wall of many invasive organisms, such as Candida albicans. In order to assess whether enolase of Plasmodium vivax is also antigenic, in this study, we first reported the expression and purification of recombinant Plasmodium vivax enolase (r-Pven) in Escherichia coli, using prokaryotic expression vector. The r-Pven was expressed in soluble form in E. coli, and the expression was verified by SDS-PAGE and western blotting analysis. The r-Pven was purified to 90% purity by nickel-nitrilotriacetic acid (Ni

Indexed as

AnimalsAntibodies, ProtozoanDisease Models, AnimalEscherichia coliHumansMalariaMalaria VaccinesMicePhosphopyruvate HydratasePlasmodium bergheiPlasmodium vivaxRecombinant ProteinsVaccines, SubunitVaccines, SyntheticAntibodies, ProtozoanMalaria VaccinesPhosphopyruvate HydrataseRecombinant ProteinsVaccines, SubunitVaccines, SyntheticEnolaseMalariaPlasmodium vivaxProkaryotic expressionVaccine candidate

Identifiers

PMID27487171
PMCPMC5137521
OpenAlexW2517099078

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.