ArticlePloS one2016
Global DNA Methylation Changes in Nile Tilapia Gonads during High Temperature-Induced Masculinization.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 98 citations in OpenAlex.
- Genome-wide DNA Methylation Difference Between Sexes in Juveniles and Epigenetic Regulation of Sexual Development in Intersex Crabs Eriocheir sinensis.Marine biotechnology (New York, N.Y.) · 2026Article
- Epigenetic Clocks Can Predict Age in the Largemouth Bass-Complex Despite Spatial and Sexual Variation.Evolutionary applications · 2026Article
- Integrative Methylome and Transcriptome Analysis of DNA Methylation and Sex-Biased Gene Expression in Northern Pike (Animals : an open access journal from MDPI · 2026Article
- Dynamic Transcriptional Regulation of the Hypothalamic-Pituitary-Testis Axis in the Seasonally Breeding TeleostInternational journal of molecular sciences · 2025Article
- Genomic and Epigenomic Influences on Resilience across Scales: Lessons from the Responses of Fish to Environmental Stressors.Integrative and comparative biology · 2024Review
- The fusion gene hsf5-rnf43 in Nile tilapia: A potential regulator in the maintenance of testis function and sexual differentiation.iScience · 2023Article
- Exploring the Effects of Rearing Densities on Epigenetic Modifications in the Zebrafish Gonads.International journal of molecular sciences · 2023Article
- Heatwave resilience of juvenile white sturgeon is associated with epigenetic and transcriptional alterations.Scientific reports · 2023Article
- Effect of high temperatures on sex ratio and differential expression analysis (RNA-seq) of sex-determining genes inEnvironmental epigenetics · 2023Article
- A Cautionary Tale of Sexing by Methylation: Hybrid Bisulfite-Conversion Sequencing of Immunoprecipitated Methylated DNA inAnimals : an open access journal from MDPI · 2022Article
- Genomic imprinting-like monoallelic paternal expression determines sex of channel catfish.Science advances · 2022Article
- Feminization of channel catfish with 17β-oestradiol involves methylation and expression of a specific set of genes independent of the sex determination region.Epigenetics · 2022Article
- Environmentally-induced sex reversal in fish with chromosomal vs. polygenic sex determination.Environmental research · 2022Article
- First account of a transient intersex in spotted scat, Scatophagus argus: a marine gonochoristic fish.Fish physiology and biochemistry · 2022Article
- DNA Methylation Difference between Female and Male Ussuri Catfish (Life (Basel, Switzerland) · 2022Article
- Comparative transcriptome analysis of heat-induced domesticated zebrafish during gonadal differentiation.BMC genomic data · 2022Article
- Identification of an Epigenetically Marked Locus within the Sex Determination Region of Channel Catfish.International journal of molecular sciences · 2022Article
- Sex-Biased miRNAs in the Gonads of Adult Chinese Alligator (Frontiers in genetics · 2022Article
- Epigenetic Regulation of Phenotypic Sexual Plasticity Inducing Skewed Sex Ratio in Zebrafish.Frontiers in cell and developmental biology · 2022Article
- Genetic and epigenetic regulation of growth, reproduction, disease resistance and stress responses in aquaculture.Frontiers in genetics · 2022Review
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Authors and funding
6 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
In some fish species, high or low temperature can switch the sex determination mechanisms and induce fish sex reversal when the gonads are undifferentiated. During this high or low temperature-induced sex reversal, the expressions of many genes are altered. However, genome-wide DNA methylation changes in fish gonads after high or low temperature treatment are unclear. Herein, we compared the global DNA methylation changes in the gonads from control females (CF), control males (CM), high temperature-treated females (TF), and high temperature-induced males (IM) from the F8 family of Nile tilapia (Oreochromis niloticus) using methylated DNA immunoprecipitation sequencing. The DNA methylation level in CF was higher than that in CM for various chromosomes. Both females and males showed an increase in methylation levels on various chromosomes after high-temperature induction. We identified 64,438 (CF/CM), 63,437 (TF/IM), 98,675 (TF/CF), 235,270 (IM/CM) and 119,958 (IM/CF) differentially methylated regions (DMRs) in Nile tilapia gonads, representing approximately 0.70% (CF/CM), 0.69% (TF/IM), 1.07% (TF/CF), 2.56% (IM/CM), and 1.30% (IM/CF)of the length of the genome. A total of 89 and 65 genes that exhibited DMRs in their gene bodies and promoters were mapped to the Nile tilapia genome. Furthermore, more than half of the genes with DMRs in the gene body in CF/CM were also included in the IM/CM, TF/CF, TF/IM, and IM/CF groups. Additionally, many important pathways, including neuroactive ligand-receptor interaction, extracellular matrix-receptor interaction, and biosynthesis of unsaturated fatty acids were identified. This study provided an important foundation to investigate the molecular mechanism of high temperature-induced sex reversal in fish species.
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