ArticleFrontiers in molecular biosciences2016
Conjugative DNA Transfer Is Enhanced by Plasmid R1 Partitioning Proteins.
Article in Frontiers in molecular biosciences, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
- Characterization of RcgA and RcgR, two rhizobial proteins involved in the modulation of plasmid transfer.Microbiology spectrum · 2026Article
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- The F plasmid conjutome: the repertoire ofmSphere · 2024Article
- Adaptive evolution of plasmid and chromosome contributes to the fitness of a blaNDM-bearing cointegrate plasmid in Escherichia coli.The ISME journal · 2024Article
- DNA Transport through the Dynamic Type IV Secretion System.Infection and immunity · 2023Review
- High Biofilm-Forming Ability and Clonal Dissemination among Colistin-ResistantLife (Basel, Switzerland) · 2023Article
- Light RegulatesJournal of bacteriology · 2022Article
- Contributions of F-specific subunits to the F plasmid-encoded type IV secretion system and F pilus.Molecular microbiology · 2022Article
- Phylogenetic Group B2 Expressed Significant Biofilm Formation among Drug Resistant Uropathogenic Escherichia coli.The Libyan journal of medicine · 2021Article
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- Alpha-Helical Protein KfrC Acts as a Switch between the Lateral and Vertical Modes of Dissemination of Broad-Host-Range RA3 Plasmid from IncU (IncP-6) Incompatibility Group.International journal of molecular sciences · 2021Article
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- Expression, Localization, and Protein Interactions of the Partitioning Proteins in the Gonococcal Type IV Secretion System.Frontiers in microbiology · 2021Article
- Comparative Genomic Analysis Uncovered Evolution of Pathogenicity Factors, Horizontal Gene Transfer Events, and Heavy Metal Resistance Traits in Citrus Canker BacteriumFrontiers in microbiology · 2021Article
- Biological and Structural Diversity of Type IV Secretion Systems.Microbiology spectrum · 2019Review
- Type IV secretion in Gram-negative and Gram-positive bacteria.Molecular microbiology · 2018Review
- Transmissible ST3-IncHI2 Plasmids Are Predominant Carriers of Diverse Complex ISFrontiers in microbiology · 2018Article
- Crosstalk between vertical and horizontal gene transfer: plasmid replication control by a conjugative relaxase.Nucleic acids research · 2017Article
- Fic Proteins ofFrontiers in microbiology · 2017Article
- Article
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Bacterial conjugation is a form of type IV secretion used to transport protein and DNA directly to recipient bacteria. The process is cell contact-dependent, yet the mechanisms enabling extracellular events to trigger plasmid transfer to begin inside the cell remain obscure. In this study of plasmid R1 we investigated the role of plasmid proteins in the initiation of gene transfer. We find that TraI, the central regulator of conjugative DNA processing, interacts physically, and functionally with the plasmid partitioning proteins ParM and ParR. These interactions stimulate TraI catalyzed relaxation of plasmid DNA in vivo and in vitro and increase ParM ATPase activity. ParM also binds the coupling protein TraD and VirB4-like channel ATPase TraC. Together, these protein-protein interactions probably act to co-localize the transfer components intracellularly and promote assembly of the conjugation machinery. Importantly these data also indicate that the continued association of ParM and ParR at the conjugative pore is necessary for plasmid transfer to start efficiently. Moreover, the conjugative pilus and underlying secretion machinery assembled in the absence of Par proteins mediate poor biofilm formation and are completely dysfunctional for pilus specific R17 bacteriophage uptake. Thus, functional integration of Par components at the interface of relaxosome, coupling protein, and channel ATPases appears important for an optimal conformation and effective activation of the transfer machinery. We conclude that low copy plasmid R1 has evolved an active segregation system that optimizes both its vertical and lateral modes of dissemination.
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