ArticleFrontiers in microbiology2016
Three CoA Transferases Involved in the Production of Short Chain Fatty Acids in Porphyromonas gingivalis.
Article in Frontiers in microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it, 30 citations in OpenAlex.
- Metabolomics Analysis as a Tool in Periodontitis Diagnosis: A Systematic Review.Clinical and experimental dental research · 2025Pooled it
- Dietary Fiber-Associated Differences in Gut Microbial Community Composition and Predicted Short-Chain Fatty Acid-Related Functional Potential: An In Silico Re-Analysis of 16S rRNA Data.International journal of molecular sciences · 2026Article
- Short-chain fatty acids-a key link between the gut microbiome and T-lymphocytes in neonates?Pediatric research · 2026Review
- Molecular Chain Elongation Mechanism for n-Caproate Biosynthesis by Megasphaera Hexanoica.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Recent Contributions of Proteomics to Our Understanding of Reversible NJournal of proteome research · 2024Review
- Redefining the coenzyme A transferase superfamily with a large set of manually annotated proteins.Protein science : a publication of the Protein Society · 2022Article
- Physiological activity of E. coli engineered to produce butyric acid.Microbial biotechnology · 2022Article
- Article
- Gut microbial indicators of metabolic health underlie age-related differences in obesity and diabetes risk among Native Hawaiians and Pacific Islanders.Frontiers in cellular and infection microbiology · 2022Article
- Gut bacteria identified in colorectal cancer patients promote tumourigenesis via butyrate secretion.Nature communications · 2021Article
- Butyryl/Caproyl-CoA:Acetate CoA-transferase: cloning, expression and characterization of the key enzyme involved in medium-chain fatty acid biosynthesis.Bioscience reports · 2021Article
- Metabolic cooperativity betweenJournal of oral microbiology · 2020Article
- Role for Lipids Secreted by Irradiated Peripheral Blood Mononuclear Cells in Inflammatory Resolution in Vitro.International journal of molecular sciences · 2020Article
- Contribution of Short Chain Fatty Acids to the Growth ofFrontiers in cellular and infection microbiology · 2020Article
- Characterization of the phosphotransacetylase-acetate kinase pathway for ATP production inJournal of oral microbiology · 2019Article
- The concordance between upper and lower respiratory microbiota in children with Mycoplasma pneumoniae pneumonia.Emerging microbes & infections · 2018Article
- Sinus Microanatomy and Microbiota in a Rabbit Model of Rhinosinusitis.Frontiers in cellular and infection microbiology · 2017Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Butyryl-CoA:acetate CoA transferase, which produces butyrate and acetyl-CoA from butyryl-CoA and acetate, is responsible for the final step of butyrate production in bacteria. This study demonstrates that in the periodontopathogenic bacterium Porphyromonas gingivalis this reaction is not catalyzed by PGN_1171, previously annotated as butyryl-CoA:acetate CoA transferase, but by three distinct CoA transferases, PGN_0725, PGN_1341, and PGN_1888. Gas chromatography/mass spectrometry (GC-MS) and spectrophotometric analyses were performed using crude enzyme extracts from deletion mutant strains and purified recombinant proteins. The experiments revealed that, in the presence of acetate, PGN_0725 preferentially utilized butyryl-CoA rather than propionyl-CoA. By contrast, this preference was reversed in PGN_1888. The only butyryl-CoA:acetate CoA transferase activity was observed in PGN_1341. Double reciprocal plots revealed that all the reactions catalyzed by these enzymes follow a ternary-complex mechanism, in contrast to previously characterized CoA transferases. GC-MS analysis to determine the concentrations of short chain fatty acids (SCFAs) in culture supernatants of P. gingivalis wild type and mutant strains revealed that PGN_0725 and PGN_1888 play a major role in the production of butyrate and propionate, respectively. Interestingly, a triple deletion mutant lacking PGN_0725, PGN_1341, and PGN_1888 produced low levels of SCFAs, suggesting that the microorganism contains CoA transferase(s) in addition to these three enzymes. Growth rates of the mutant strains were mostly slower than that of the wild type, indicating that many carbon compounds produced in the SCFA synthesis appear to be important for the biological activity of this microorganism.
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