ArticleReproduction (Cambridge, England)2016
Rhox13 is required for a quantitatively normal first wave of spermatogenesis in mice.
Article in Reproduction (Cambridge, England), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 34 citations in OpenAlex.
- Gene regulatory networks orchestrating oocyte fate bifurcation in primordial follicles revealed by single-cell transcriptomics.Communications biology · 2026Article
- Transcriptomic dynamics and cell-to-cell communication during the transition of prospermatogonia to spermatogonia revealed at single-cell resolution.BMC genomics · 2025Article
- Article
- Epigenetic priming in the male germline.Current opinion in genetics & development · 2024Review
- Article
- Autophagy regulation and protein kinase activity of PIK3C3 controls sertoli cell polarity through its negative regulation on SCIN (scinderin).Autophagy · 2023Article
- Rhox8 homeobox gene ablation leads to rete testis abnormality and male subfertility in mice†.Biology of reproduction · 2023Article
- Mammal Reproductive Homeobox (Rhox) Genes: An Update of Their Involvement in Reproduction and Development.Genes · 2023Review
- Review
- Do macrophages play a role in the adverse effects of endocrine disrupting chemicals (EDCs) on testicular functions?Frontiers in toxicology · 2023Review
- Dual DNA staining enables isolation of multiple sub-types of post-replicative mouse male germ cells.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2022Article
- Concordant Androgen-Regulated Expression of Divergent Rhox5 Promoters in Sertoli Cells.Endocrinology · 2022Article
- Aurora A Kinase (AURKA) is required for male germline maintenance and regulates sperm motility in the mouse.Biology of reproduction · 2021Article
- RHOX10 drives mouse spermatogonial stem cell establishment through a transcription factor signaling cascade.Cell reports · 2021Article
- TheProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Testis-expressed protein 33 is not essential for spermiogenesis and fertility in mice.Molecular medicine reports · 2021Article
- Targeted disruption of galectin 3 in mice delays the first wave of spermatogenesis and increases germ cell apoptosis.Cellular and molecular life sciences : CMLS · 2021Article
- A framework for high-resolution phenotyping of candidate male infertility mutants: from human to mouse.Human genetics · 2021Review
- Epigenetic Effects Promoted by Neonicotinoid Thiacloprid Exposure.Frontiers in cell and developmental biology · 2021Article
- The rapamycin analog Everolimus reversibly impairs male germ cell differentiation and fertility in the mouse†.Biology of reproduction · 2020Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
We previously described a novel germ cell-specific X-linked reproductive homeobox gene (Rhox13) that is upregulated at the level of translation in response to retinoic acid (RA) in differentiating spermatogonia and preleptotene spermatocytes. We hypothesize that RHOX13 plays an essential role in male germ cell differentiation, and have tested this by creating a Rhox13 gene knockout (KO) mouse. Rhox13 KO mice are born in expected Mendelian ratios, and adults have slightly reduced testis weights, yet a full complement of spermatogenic cell types. Young KO mice (at ~7-8 weeks of age) have a ≈50% reduction in epididymal sperm counts, but numbers increased to WT levels as the mice reach ~17 weeks of age. Histological analysis of testes from juvenile KO mice reveals a number of defects during the first wave of spermatogenesis. These include increased apoptosis, delayed appearance of round spermatids and disruption of the precise stage-specific association of germ cells within the seminiferous tubules. Breeding studies reveal that both young and aged KO males produce normal-sized litters. Taken together, our results indicate that RHOX13 is not essential for mouse fertility in a controlled laboratory setting, but that it is required for optimal development of differentiating germ cells and progression of the first wave of spermatogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.