ArticleScientific reports2016
Dynamic mRNA and miRNA expression analysis in response to intermuscular bone development of blunt snout bream (Megalobrama amblycephala).
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 47 citations in OpenAlex.
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- Creation of intermuscular bone-free genetic mutants in grass carp and multiomics reveals molecular regulatory basis.Science China. Life sciences · 2026Article
- Fgf8, a gene knockout that leads to intermuscular bones-reduced of crucian carp (Carassius auratus), acts as a potentially regulatory factor in osteogenic development.Fish physiology and biochemistry · 2026Article
- Molecular mechanisms of intermuscular bone development in Acrossocheilus wenchowensis.Journal of fish biology · 2026Article
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- Full-length transcriptome assembly of black amur bream (Megalobrama terminalis) as a reference resource.Molecular biology reports · 2024Article
- Decoding the fish genome opens a new era in important trait research and molecular breeding in China.Science China. Life sciences · 2024Review
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- Single-cell transcriptomes andNational science review · 2022Review
- Differentially Expressed miRNAs and mRNAs in Regenerated Scales of Rainbow Trout (Animals : an open access journal from MDPI · 2022Article
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- Dynamic mRNA and miRNA expression of the head during early development in bighead carp (Hypophthalmichthys nobilis).BMC genomics · 2022Article
- Transcriptomic Analysis Provides Insights to Reveal theFrontiers in cell and developmental biology · 2022Article
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- Genome-Wide Integrated Analysis Revealed Functions of lncRNA-miRNA-mRNA Interaction in Growth of Intermuscular Bones inFrontiers in cell and developmental biology · 2020Article
- A Comparative Genomic and Transcriptional Survey Providing Novel Insights into Bone Morphogenetic Protein 2 (International journal of molecular sciences · 2019Article
- Development of Teleost Intermuscular Bones Undergoing Intramembranous Ossification Based on Histological-Transcriptomic-Proteomic Data.International journal of molecular sciences · 2019Article
- Evolution of the intermuscular bones in the Cyprinidae (Pisces) from a phylogenetic perspective.Ecology and evolution · 2019Article
- Integrated Analysis of Transcriptomic, miRNA and Proteomic Changes of a Novel Hybrid Yellow Catfish Uncovers Key Roles for miRNAs in Heterosis.Molecular & cellular proteomics : MCP · 2019Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Intermuscular bone (IB), which occurs only in the myosepta of lower teleosts, is attracting more attention because they are difficult to remove and make the fish unpleasant to eat. By gaining a better understanding of the genetic regulation of IB development, an integrated analysis of miRNAs and mRNAs expression profiling was performed on Megalobrama amblycephala. Four key development stages were selected for transcriptome and small RNA sequencing. A number of significantly differentially expressed miRNAs/genes associated with bone formation and differentiation were identified and the functional characteristics of these miRNAs/genes were revealed by GO function and KEGG pathway analysis. These were involved in TGF-β, ERK and osteoclast differentiation pathways known in the literature to affect bone formation and differentiation. MiRNA-mRNA interaction pairs were detected from comparison of expression between different stages. The function annotation results also showed that many miRNA-mRNA interaction pairs were likely to be involved in regulating bone development and differentiation. A negative regulation effect of two miRNAs was verified through dual luciferase reporter assay. As a unique public resource for gene expression and regulation during the IB development, this study is expected to provide forwards ideas and resources for further biological researches to understand the IBs' development.
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