ArticleNeurochemical research2016
Uptake and Toxicity of Copper Oxide Nanoparticles in C6 Glioma Cells.
Article in Neurochemical research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 47 citations in OpenAlex.
- Application of nanoparticles in breast cancer treatment: a systematic review.Naunyn-Schmiedeberg's archives of pharmacology · 2024Pooled it
- Emerging role of copper in the pathophysiology of spinal cord injury.Neural regeneration research · 2026Article
- Zinc and Copper Metallic Instability: Investigating Altered Metal Functionality in both Human and Animal Studies.Biological trace element research · 2026Review
- Impact of particle size and surface modifications on the neurotoxic potential of copper oxide nanoparticles.Scientific reports · 2025Article
- The Pattern of Copper Release in Copper-Based Nanoparticles Regulates Tumor Proliferation and Invasiveness in 3D Culture Models.Small science · 2024Article
- Copper homeostasis and cuproptosis in central nervous system diseases.Cell death & disease · 2024Review
- CuO nanoparticles for glioma treatment in vitro and in vivo.Scientific reports · 2024Article
- Evolving approaches in glioma treatment: harnessing the potential of copper metabolism modulation.RSC advances · 2023Review
- Targeting copper death genotyping associated gene RARRES2 suppresses glioblastoma progression and macrophages infiltration.Cancer cell international · 2023Article
- Identification of a copper metabolism-related gene signature for predicting prognosis and immune response in glioma.Cancer medicine · 2023Article
- Role of inorganic nanoparticle degradation in cancer therapy.Nanoscale advances · 2020Review
- Cupric Oxide Nanoparticles Induce Cellular Toxicity in Liver and Intestine Cell Lines.Advanced pharmaceutical bulletin · 2020Article
- Iron-Doping of Copper Oxide Nanoparticles Lowers Their Toxic Potential on C6 Glioma Cells.Neurochemical research · 2020Article
- Model-Based Nanoengineered Pharmacokinetics of Iron-Doped Copper Oxide for Nanomedical Applications.Angewandte Chemie (International ed. in English) · 2020Article
- Uptake of Intact Copper Oxide Nanoparticles Causes Acute Toxicity in Cultured Glial Cells.Neurochemical research · 2019Article
- How to Study the Uptake and Toxicity of Nanoparticles in Cultured Brain Cells: The Dos and Don't Forgets.Neurochemical research · 2019Review
- Monitoring of the Cytoskeleton-Dependent Intracellular Trafficking of Fluorescent Iron Oxide Nanoparticles by Nanoparticle Pulse-Chase Experiments in C6 Glioma Cells.Neurochemical research · 2018Article
- High copper concentrations produce genotoxicity and cytotoxicity in bovine cumulus cells.Environmental science and pollution research international · 2017Article
- The roles of surface chemistry, dissolution rate, and delivered dose in the cytotoxicity of copper nanoparticles.Nanoscale · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Copper oxide nanoparticles (CuO-NPs) are frequently used for many technical applications, but are also known for their cell toxic potential. In order to investigate a potential use of CuO-NPs as a therapeutic drug for glioma treatment, we have investigated the consequences of an application of CuO-NPs on the cellular copper content and cell viability of C6 glioma cells. CuO-NPs were synthesized by a wet-chemical method and were coated with dimercaptosuccinic acid and bovine serum albumin to improve colloidal stability in physiological media. Application of these protein-coated nanoparticles (pCuO-NPs) to C6 cells caused a strong time-, concentration- and temperature-dependent copper accumulation and severe cell death. The observed loss in cellular MTT-reduction capacity, the loss in cellular LDH activity and the increase in the number of propidium iodide-positive cells correlated well with the specific cellular copper content. C6 glioma cells were less vulnerable to pCuO-NPs compared to primary astrocytes and toxicity of pCuO-NPs to C6 cells was only observed for incubation conditions that increased specific cellular copper contents above 20 nmol copper per mg protein. Both cellular copper accumulation as well as the pCuO-NP-induced toxicity in C6 cells were prevented by application of copper chelators, but not by endocytosis inhibitors, suggesting that liberation of copper ions from the pCuO-NPs is the first step leading to the observed toxicity of pCuO-NP-treated glioma cells.
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