ArticleScientific reports2016
Macromolecular crowding explains overflow metabolism in cells.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed.
- Thermo-flux: generation and analysis of thermodynamic-stoichiometric metabolic network models.Molecular systems biology · 2026Article
- Cell geometry and membrane protein crowding constrain Escherichia coli growth rate, overflow metabolism, respiration, and maintenance energy.FEBS letters · 2026Article
- On the conditions for shifts in metabolic strategies.PLoS computational biology · 2026Review
- Acetate-Linked Energy Metabolism as a Determinant of Early Haemophilus influenzae Infection Fitness.MicrobiologyOpen · 2026Article
- Overflow metabolism in bacterial, yeast, and mammalian cells: different names, same game.Molecular systems biology · 2025Review
- Review
- Cell Geometry Limits Bacterial Metabolic Efficiency.bioRxiv : the preprint server for biology · 2025Article
- Cell Geometry and Membrane Protein Crowding Constrain Growth Rate, Overflow Metabolism, Respiration, and Maintenance Energy.bioRxiv : the preprint server for biology · 2025Article
- Article
- Metabolic footprint and logic through the T cell life cycle.Current opinion in immunology · 2024Review
- Osmolarity-Induced Altered Intracellular Molecular Crowding Drives Osteoarthritis Pathology.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- The Warburg Effect Reinterpreted 100 yr on: A First-Principles Stoichiometric Analysis and Interpretation from the Perspective of ATP Metabolism in Cancer Cells.Function (Oxford, England) · 2024Article
- Macromolecular crowding: Sensing without a sensor.Current opinion in cell biology · 2023Review
- Inference of metabolic fluxes in nutrient-limited continuous cultures: A Maximum Entropy approach with the minimum information.iScience · 2022Article
- Article
- Progress on Crowding Effect in Cell-like Structures.Membranes · 2022Review
- Is energy excess the initial trigger of carbon overflow metabolism? Transcriptional network response of carbon-limited Escherichia coli to transient carbon excess.Microbial cell factories · 2022Article
- Perspective: a stirring role for metabolism in cells.Molecular systems biology · 2022Review
- Article
- Review
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2 authors.
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Abstract
Overflow metabolism is a metabolic phenotype of cells characterized by mixed oxidative phosphorylation (OxPhos) and fermentative glycolysis in the presence of oxygen. Recently, it was proposed that a combination of a protein allocation constraint and a higher proteome fraction cost of energy generation by OxPhos relative to fermentation form the basis of overflow metabolism in the bacterium, Escherichia coli. However, we argue that the existence of a maximum or optimal macromolecular density is another essential requirement. Here we re-evaluate our previous theory of overflow metabolism based on molecular crowding following the proteomic fractions formulation. We show that molecular crowding is a key factor in explaining the switch from OxPhos to overflow metabolism.
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