ArticlePloS one2016
A Single-Batch Fermentation System to Simulate Human Colonic Microbiota for High-Throughput Evaluation of Prebiotics.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed.
- Effects of Rich in Β-Glucans Edible Mushrooms on Aging Gut Microbiota Characteristics: An In Vitro Study.Molecules (Basel, Switzerland) · 2020Trial
- Microbes and medicines: interrelationships between pharmaceuticals and the gut microbiome.Gut microbes · 2026Review
- Demystifying the role of postbiotics in inflammation mediated metabolic disorders: an updated review.Food science and biotechnology · 2026Review
- Microbiome-aware oral drug delivery: excipient-microbiome interactions and nanocarrier design rules for predictable formulation performance.Frontiers in pharmacology · 2026Review
- Function-based personalization of dietary fiber selection enhances gut microbial fermentation: a proof-of-concept crossover trial.Frontiers in nutrition · 2026Article
- Fecal Short-Chain Fatty Acids in Colorectal Cancer Patients Versus Healthy Controls: A Systematic Review and Meta-Analysis.Journal of clinical medicine · 2025Review
- Effects of Postbiotic Administration on Canine Health: A Systematic Review and Meta-Analysis.Microorganisms · 2025Review
- Promoting the Emerging Role of Pulse By-Products as Valuable Sources of Functional Compounds and Novel Food Ingredients.Foods (Basel, Switzerland) · 2025Review
- Health Benefits and Safety of Postbiotics Derived from Different Probiotic Species.Current pharmaceutical design · 2025Review
- Review
- An In Vitro Colonic Fermentation Study of the Effects of Human Milk Oligosaccharides on Gut Microbiota and Short-Chain Fatty Acid Production in Infants Aged 0-6 Months.Foods (Basel, Switzerland) · 2024Article
- Establishment of porcine fecal-derivedBioscience of microbiota, food and health · 2024Article
- Effects of blackcurrant extract on indole and ammonia productions in anBioscience of microbiota, food and health · 2024Article
- Exploring the Potential of Postbiotics for Food Safety and Human Health Improvement.Journal of nutrition and metabolism · 2024Review
- Postbiotics: Functional Food Materials and Therapeutic Agents for Cancer, Diabetes, and Inflammatory Diseases.Foods (Basel, Switzerland) · 2023Review
- Effects of SynbioticFoods (Basel, Switzerland) · 2023Article
- Sulfated and non-sulfated chondroitin affect the composition and metabolism of human colonic microbiota simulated in an in vitro fermentation system.Scientific reports · 2023Article
- Prebiotic and Anti-Adipogenic Effects of Radish Green Polysaccharide.Microorganisms · 2023Article
- A short-term bioreactor assay to assess the effect of essential oils on a microbiota derived from piglet's intestinal content.Acta veterinaria Scandinavica · 2023Article
- Fermentation Supernatant of Elderly Feces with Inulin and Partially Hydrolyzed Guar Gum Maintains the Barrier of Inflammation-Induced Caco-2/HT29-MTX-E12 Co-Cultured Cells.Journal of agricultural and food chemistry · 2023Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We devised a single-batch fermentation system to simulate human colonic microbiota from fecal samples, enabling the complex mixture of microorganisms to achieve densities of up to 1011 cells/mL in 24 h. 16S rRNA gene sequence analysis of bacteria grown in the system revealed that representatives of the major phyla, including Bacteroidetes, Firmicutes, and Actinobacteria, as well as overall species diversity, were consistent with those of the original feces. On the earlier stages of fermentation (up to 9 h), trace mixtures of acetate, lactate, and succinate were detectable; on the later stages (after 24 h), larger amounts of acetate accumulated along with some of propionate and butyrate. These patterns were similar to those observed in the original feces. Thus, this system could serve as a simple model to simulate the diversity as well as the metabolism of human colonic microbiota. Supplementation of the system with several prebiotic oligosaccharides (including fructo-, galacto-, isomalto-, and xylo-oligosaccharides; lactulose; and lactosucrose) resulted in an increased population in genus Bifidobacterium, concomitant with significant increases in acetate production. The results suggested that this fermentation system may be useful for in vitro, pre-clinical evaluation of the effects of prebiotics prior to testing in humans.
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