ArticleThe ISME journal2017
Metal stressors consistently modulate bacterial conjugal plasmid uptake potential in a phylogenetically conserved manner.
Article in The ISME journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 157 citations in OpenAlex.
- Prolonged zinc exposure modulates biofilm metabolic activity and conjugation in Enterococcus faecalis.BMC microbiology · 2026Article
- Selective shifts in mobile antibiotic resistance genes under carbamazepine exposure in wastewater microbiomes.ISME communications · 2026Article
- Roles of micro/nanoplastics in the spread of antimicrobial resistance through conjugative gene transfer.Nature communications · 2025Article
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- Impact of fluoroquinolone and heavy metal pollution on antibiotic resistance maintenance in aquatic ecosystems.Environmental microbiome · 2025Article
- Effects of Cigarette-Derived Compounds on the Spread of Antimicrobial Resistance in Artificial Human Lung Sputum Medium, Simulated Environmental Media, and Wastewater.Environmental health perspectives · 2025Article
- Jellyfish blooms-an overlooked hotspot and potential vector for the transmission of antimicrobial resistance in marine environments.mSystems · 2025Article
- Dynamic Spread of Antibiotic Resistance Determinants by Conjugation to a Human-Derived Gut Microbiota in a Transplanted Mouse Model.Antibiotics (Basel, Switzerland) · 2025Article
- Article
- Ecology-based approach to predict no-effect antibiotic concentrations for minimizing environmental selection of resistance.The ISME journal · 2025Article
- Biochar suppresses conjugative transfer of antibiotic resistance genes in manure-amended soils.The ISME journal · 2025Article
- Mating Assay: Plating Below a Cell Density Threshold is Required for Unbiased Estimation of Plasmid Conjugation Frequency of RP4 Transfer Between E. coli Strains.Microbial ecology · 2024Article
- Environmental microbiome diversity and stability is a barrier to antimicrobial resistance gene accumulation.Communications biology · 2024Article
- Evolution of triclosan resistance modulates bacterial permissiveness to multidrug resistance plasmids and phages.Nature communications · 2024Article
- Current examining methods and mathematical models of horizontal transfer of antibiotic resistance genes in the environment.Frontiers in microbiology · 2024Review
- Mixed waste contamination selects for a mobile genetic element population enriched in multiple heavy metal resistance genes.ISME communications · 2024Article
- Plasmids, a molecular cornerstone of antimicrobial resistance in the One Health era.Nature reviews. Microbiology · 2024Review
- Effect of Zinc Oxide and Copper Sulfate on Antibiotic Resistance Plasmid Transfer inMicroorganisms · 2023Article
- Article
- Unrecognized risk of perfluorooctane sulfonate in promoting conjugative transfers of bacterial antibiotic resistance genes.Applied and environmental microbiology · 2023Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
The environmental stimulants and inhibitors of conjugal plasmid transfer in microbial communities are poorly understood. Specifically, it is not known whether exposure to stressors may cause a community to alter its plasmid uptake ability. We assessed whether metals (Cu, Cd, Ni, Zn) and one metalloid (As), at concentrations causing partial growth inhibition, modulate community permissiveness (that is, uptake ability) against a broad-host-range IncP-type plasmid (pKJK5). Cells were extracted from an agricultural soil as recipient community and a cultivation-minimal filter mating assay was conducted with an exogenous E. coli donor strain. The donor hosted a gfp-tagged pKJK5 derivative from which conjugation events could be microscopically quantified and transconjugants isolated and phylogenetically described at high resolution via FACS and 16S rRNA amplicon sequencing. Metal stress consistently decreased plasmid transfer frequencies to the community, while the transconjugal pool richness remained unaffected with OTUs belonging to 12 bacterial phyla. The taxonomic composition of the transconjugal pools was distinct from their respective recipient communities and clustered dependent on the stress type and dose. However, for certain OTUs, stress increased or decreased permissiveness by more than 1000-fold and this response was typically correlated across different metals and doses. The response to some stresses was, in addition, phylogenetically conserved. This is the first demonstration that community permissiveness is sensitive to metal(loid) stress in a manner that is both partially consistent across stressors and phylogenetically conserved.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.