ArticleThe Plant journal : for cell and molecular biology2016
The impact of mechanical compression on cortical microtubules in Arabidopsis: a quantitative pipeline.
Article in The Plant journal : for cell and molecular biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 46 citations in OpenAlex.
- Hydraulic Signaling in Plants: From Physical Perturbation to Distributed Perception and Context-Dependent Decoding.Plants (Basel, Switzerland) · 2026Review
- ROS inhibits microtubule dynamics and cell growth heterogeneity during Arabidopsis sepal morphogenesis.iScience · 2026Article
- From Pixels to Patterns: A Multidimensional Framework to Decode Cytoskeletal Organization.Computational and structural biotechnology journal · 2026Review
- Tubulin Acetylation and the Cellular Mechanosensing and Stress Response.Results and problems in cell differentiation · 2025Review
- Tubulin Acetylation: A Critical Regulator of Microtubule Function.Results and problems in cell differentiation · 2025Review
- Mechanical forces in plant growth and development.Journal of plant research · 2024Article
- Spatiotemporally distinct responses to mechanical forces shape the developing seed of Arabidopsis.The EMBO journal · 2024Article
- Can repetitive mechanical motion cause structural damage to axons?Frontiers in molecular neuroscience · 2024Review
- High-throughput characterization of cortical microtubule arrays response to anisotropic tensile stress.BMC biology · 2023Article
- Mechanical Forces in Floral Development.Plants (Basel, Switzerland) · 2022Review
- Cell biology of the leaf epidermis: Fate specification, morphogenesis, and coordination.The Plant cell · 2022Review
- IQ67 DOMAIN proteins facilitate preprophase band formation and division-plane orientation.Nature plants · 2021Article
- GR24, A Synthetic Strigolactone Analog, and Light Affect the Organization of Cortical Microtubules in Arabidopsis Hypocotyl Cells.Frontiers in plant science · 2021Article
- Plant cell divisions: variations from the shortest symmetric path.Biochemical Society transactions · 2020Review
- Article
- Microtubule self-organisation during seed germination in Arabidopsis.BMC biology · 2020Article
- Calcium signals are necessary to establish auxin transporter polarity in a plant stem cell niche.Nature communications · 2019Article
- Mechanical Conflicts in Twisting Growth Revealed by Cell-Cell Adhesion Defects.Frontiers in plant science · 2019Article
- Quantitative analysis of cell shape and the cytoskeleton in developmental biology.Wiley interdisciplinary reviews. Developmental biology · 2018Review
- Abiotic Stress Signaling in Wheat - An Inclusive Overview of Hormonal Interactions During Abiotic Stress Responses in Wheat.Frontiers in plant science · 2018Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Exogenous mechanical perturbations on living tissues are commonly used to investigate whether cell effectors can respond to mechanical cues. However, in most of these experiments, the applied mechanical stress and/or the biological response are described only qualitatively. We developed a quantitative pipeline based on microindentation and image analysis to investigate the impact of a controlled and prolonged compression on microtubule behaviour in the Arabidopsis shoot apical meristem, using microtubule fluorescent marker lines. We found that a compressive stress, in the order of magnitude of turgor pressure, induced apparent microtubule bundling. Importantly, that response could be reversed several hours after the release of compression. Next, we tested the contribution of microtubule severing to compression-induced bundling: microtubule bundling seemed less pronounced in the katanin mutant, in which microtubule severing is dramatically reduced. Conversely, some microtubule bundles could still be observed 16 h after the release of compression in the spiral2 mutant, in which severing rate is instead increased. To quantify the impact of mechanical stress on anisotropy and orientation of microtubule arrays, we used the nematic tensor based FibrilTool ImageJ/Fiji plugin. To assess the degree of apparent bundling of the network, we developed several methods, some of which were borrowed from geostatistics. The final microtubule bundling response could notably be related to tissue growth velocity that was recorded by the indenter during compression. Because both input and output are quantified, this pipeline is an initial step towards correlating more precisely the cytoskeleton response to mechanical stress in living tissues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.