ArticleBiochemistry and biophysics reports2016
Electroporation by subnanosecond pulses.
Article in Biochemistry and biophysics reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 29 citations in OpenAlex.
- Electrical Stimulation of Cells: Drivers, Technology, and Effects.Chemical reviews · 2025Review
- A review with updated perspectives on in vitro and in vivo wound healing models.Turkish journal of biology = Turk biyoloji dergisi · 2023Article
- The interplay of excitation and electroporation in nanosecond pulse stimulation.Bioelectrochemistry (Amsterdam, Netherlands) · 2020Review
- Probing Nanoelectroporation and Resealing of the Cell Membrane by the Entry of CaInternational journal of molecular sciences · 2020Article
- Cancellation of nerve excitation by the reversal of nanosecond stimulus polarity and its relevance to the gating time of sodium channels.Cellular and molecular life sciences : CMLS · 2019Article
- Genome mutation after introduction of the gene editing by electroporation of Cas9 protein (GEEP) system in matured oocytes and putative zygotes.In vitro cellular & developmental biology. Animal · 2019Article
- Cell Fragmentation and Permeabilization by a 1 ns Pulse Driven Triple-Point Electrode.BioMed research international · 2018Article
- Damage-free peripheral nerve stimulation by 12-ns pulsed electric field.Scientific reports · 2017Article
- Neuronal excitation and permeabilization by 200-ns pulsed electric field: An optical membrane potential study with FluoVolt dye.Biochimica et biophysica acta. Biomembranes · 2017Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Electropermeabilization of cell membranes by micro- and nanosecond-duration stimuli has been studied extensively, whereas effects of picosecond electric pulses (psEP) remain essentially unexplored. We utilized whole-cell patch clamp and Di-8-ANEPPS voltage-sensitive dye measurements to characterize plasma membrane effects of 500 ps stimuli in rat hippocampal neurons (RHN), NG108, and CHO cells. Even a single 500-ps pulse at 190 kV/cm increased membrane conductance and depolarized cells. These effects were augmented by applying brief psEP bursts (5-125 pulses), whereas the rate of pulse delivery (8Hz - 1 kHz) played little role. psEP-treated cells displayed large inward current at negative membrane potentials but modest or no conductance changes at positive potentials. A 1-kHz burst of 25 pulses increased the whole-cell conductance in the range (-100) - (-60) mV to 22-26 nS in RHN and NG108 cells (from 3 and 0.7 nS, respectively), but only to 5 nS in CHO (from 0.3 nS). The conductance increase was reversible within about 2 min. Such pattern of cell permeabilization, with characteristic inward rectification and slow recovery, was similar to earlier reported effects of 60- and 600-ns pulses, pointing to the similarity of structural membrane rearrangements in spite of a different membrane charging mechanism.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.