ArticleJournal of biological methods
Sample preparation for proteomic analysis using a GeLC-MS/MS strategy.
Article in Journal of biological methods. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
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- Comparative Analysis of Digestion Methods for Bile Proteomics: The Key to Unlocking Biliary Biomarker Potential.Analytical chemistry · 2024Article
- Top-Down Proteomics and Comparative 2D-DIGE Analysis.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Concurrent inhibition of CDK2 adds to the anti-tumour activity of CDK4/6 inhibition in GIST.British journal of cancer · 2022Article
- The fission yeast FLCN/FNIP complex augments TORC1 repression or activation in response to amino acid (AA) availability.iScience · 2021Article
- Mechanism of p38 MAPK-induced EGFR endocytosis and its crosstalk with ligand-induced pathways.The Journal of cell biology · 2021Article
- Proteomics of broad deubiquitylase inhibition unmasks redundant enzyme function to reveal substrates and assess enzyme specificity.Cell chemical biology · 2021Article
- Erythromycin Treatment ofLife (Basel, Switzerland) · 2020Article
- Utility of Proteomics in Emerging and Re-Emerging Infectious Diseases Caused by RNA Viruses.Journal of proteome research · 2020Review
- PHD3 Loss Promotes Exercise Capacity and Fat Oxidation in Skeletal Muscle.Cell metabolism · 2020Article
- Seminal Plasma Proteome as an Indicator of Sperm Dysfunction and Low Sperm Motility in Chickens.Molecular & cellular proteomics : MCP · 2020Article
- Cdk1 Controls Global Epigenetic Landscape in Embryonic Stem Cells.Molecular cell · 2020Article
- Native Chromatin Proteomics Reveals a Role for Specific Nucleoporins in Heterochromatin Organization and Maintenance.Molecular cell · 2020Article
- Dynamics of Non-Canonical Amino Acid-Labeled Intra- and Extracellular Proteins in the Developing Mouse.Cellular and molecular bioengineering · 2019Article
- Secretomics to Discover Regulators in Diseases.International journal of molecular sciences · 2019Review
- Proteome Analysis Using Gel-LC-MS/MS.Current protocols in protein science · 2019Article
- Systematic proteomics of endogenous human cohesin reveals an interaction with diverse splicing factors and RNA-binding proteins required for mitotic progression.The Journal of biological chemistry · 2019Article
- Lysine-specific demethylase 2A enhances binding of various nuclear factors to CpG-rich genomic DNAs by action of its CXXC-PHD domain.Scientific reports · 2019Article
- Utilization of dried and long-term stored polyacrylamide gels for the advanced proteomic profiling of mitochondrial contact sites from rat liver.Biology methods & protocols · 2018Article
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Abstract
In-gel digestion coupled with mass spectrometric analysis (GeLC-MS/MS) is a cornerstone for protein identification and characterization. Here I review this versatile approach which combines classical and modern biochemistry strategies and allows for targeted and proteome-wide analyses. Starting with any protein sample, reduced and alkylated proteins are precipitated prior to fractionation by SDS-PAGE. Proteins are in-gel digested and the resulting peptides are extracted and desalted for downstream LC-MS/MS analysis. GeLC-MS/MS leverages the advantages of both traditional SDS-PAGE visualization and protein fractionation with the robust protein and post-translational modification identification and quantitation capabilities of state-of-the-art mass spectrometry-based technology. As such, this strategy allows for the visible assessment of protein amount and quality, prior to analysis via virtually any mass spectrometry platform. Moreover, gel extracted peptides may be derived from any sample type-
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