ArticleJournal of bacteriology2016
Identification and Characterization of a Putative Manganese Export Protein in Vibrio cholerae.
Article in Journal of bacteriology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 24 citations in OpenAlex.
- MneA and TerC exhibit distinct roles in manganese detoxification and virulence inApplied and environmental microbiology · 2026Article
- Study of excess manganese stress response highlights the central role of manganese exporter Mnx for holding manganese homeostasis in the cyanobacteriumMicrobiology (Reading, England) · 2024Article
- Functional characterization of a TerC family protein ofApplied and environmental microbiology · 2024Article
- Functional characterization of two TolC in the resistance to drugs and metals and in the virulence ofApplied and environmental microbiology · 2023Article
- Manganese Efflux Achieved by MetA and MetB Affects Oxidative Stress Resistance and Iron Homeostasis in Riemerella anatipestifer.Applied and environmental microbiology · 2023Article
- New insights into the pathogenicity of TMEM165 variants using structural modeling based on AlphaFold 2 predictions.Computational and structural biotechnology journal · 2023Article
- Why is manganese so valuable to bacterial pathogens?Frontiers in cellular and infection microbiology · 2023Review
- Development of bacterial resistance induced by low concentration of two-dimensional black phosphorusRSC advances · 2022Article
- A Golgi-localized manganese transporter functions in pollen tube tip growth to control male fertility inPlant communications · 2021Article
- A multifaceted cellular damage repair and prevention pathway promotes high-level tolerance to β-lactam antibiotics.EMBO reports · 2021Article
- Gene Replacement in Arabidopsis Reveals Manganese Transport as an Ancient Feature of Human, Plant and Cyanobacterial UPF0016 Proteins.Frontiers in plant science · 2021Article
- From the Uncharacterized Protein Family 0016 to the GDT1 family: Molecular insights into a newly-characterized family of cation secondary transporters.Microbial cell (Graz, Austria) · 2020Review
- Bacterial manganese sensing and homeostasis.Current opinion in chemical biology · 2020Review
- Vibrio cholerae Virulence Activator ToxR Regulates Manganese Transport and Resistance to Reactive Oxygen Species.Infection and immunity · 2020Article
- The yeast protein Gdt1p transports MnThe Journal of biological chemistry · 2018Article
- Structure-function analysis of manganese exporter proteins across bacteria.The Journal of biological chemistry · 2018Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
unlabelledManganese plays an important role in the cellular physiology and metabolism of bacterial species, including the human pathogen Vibrio cholerae The intracellular level of manganese ions is controlled through coordinated regulation of the import and export of this element. We have identified a putative manganese exporter (VC0022), named mneA (manganese exporter A), which is highly conserved among Vibrio spp. An mneA mutant exhibited sensitivity to manganese but not to other cations. Under high-manganese conditions, the mneA mutant showed an almost 50-fold increase in intracellular manganese levels and reduced intracellular iron relative to those of its wild-type parent, suggesting that the mutant's manganese sensitivity is due to the accumulation of toxic levels of manganese and reduced iron. Expression of mneA suppressed the manganese-sensitive phenotype of an Escherichia coli strain carrying a mutation in the nonhomologous manganese export gene, mntP, further supporting a manganese export function for V. cholerae MneA. The level of mneA mRNA was induced approximately 2.5-fold after addition of manganese to the medium, indicating regulation of this gene by manganese. This study offers the first insights into understanding manganese homeostasis in this important pathogen. IMPORTANCE: Bacterial cells control intracellular metal concentrations by coordinating acquisition in metal-limited environments with export in metal-excess environments. We identified a putative manganese export protein, MneA, in Vibrio cholerae An mneA mutant was sensitive to manganese, and this effect was specific to manganese. The mneA mutant accumulated high levels of intracellular manganese with a concomitant decrease in intracellular iron levels when grown in manganese-supplemented medium. Expression of mneA in trans suppressed the manganese sensitivity of an E. coli mntP mutant. This study is the first to investigate manganese export in V. cholerae.
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Registered trials
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