Evidence map›Paper›PMID 27481514›Full record

ArticleTumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine2016

Oral pathogens change proliferation properties of oral tumor cells by affecting gene expression of human defensins.

T Hoppe, D Kraus, N Novak, R Probstmeier, M Frentzen, M Wenghoefer, S Jepsen, J Winter

Abstract readComparative Study
PubMed Publisher
In one paragraph

Article in Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed, 1 pooled it
2.8field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 1 synthesis or guideline pooled it, 61 citations in OpenAlex.

  1. Pooled it
  2. The Role ofCancers · 2025
    Review
  3. Review
  4. Article
  5. Review
  6. Review
  7. Review
  8. Article
  9. Review
  10. Review
  11. Article
  12. Review
  13. The Role of IL-17 in the Pathogenesis of Oral Squamous Cell Carcinoma.International journal of molecular sciences · 2023
    Review
  14. Review
  15. Review
  16. Armed to the Teeth-The Oral Mucosa Immunity System and Microbiota.International journal of molecular sciences · 2022
    Review
  17. Alterations in Immune-Related Defensin Alpha 4 (International journal of inflammation · 2022
    Review
  18. Role of Hydrogen Sulfide in Oral Disease.Oxidative medicine and cellular longevity · 2022
    Review
  19. Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

T HoppeDepartment of Periodontology, Operative and Preventive Dentistry, University of Bonn, Welschnonnenstr. 17, 53111, Bonn, Germany.
D KrausDepartment of Prosthodontics, Preclinical Education, and Material Science, University of Bonn, Welschnonnenstr. 17, 53111, Bonn, Germany.
N NovakDepartment of Dermatology and Allergy, University Hospital Bonn, University of Bonn, Sigmund-Freud-Str. 25, 53127, Bonn, Germany.
R ProbstmeierNeuro- and Tumor Cell Biology Group, Department of Nuclear Medicine, University Hospital Bonn, University of Bonn, Sigmund-Freud-Str. 25, 53127, Bonn, Germany.
M FrentzenDepartment of Periodontology, Operative and Preventive Dentistry, University of Bonn, Welschnonnenstr. 17, 53111, Bonn, Germany.
M WenghoeferDepartment of Oral and Maxillofacial Plastic Surgery, University Hospital Bonn, University of Bonn, Sigmund-Freud-Str. 25, 53127, Bonn, Germany.
S JepsenDepartment of Periodontology, Operative and Preventive Dentistry, University of Bonn, Welschnonnenstr. 17, 53111, Bonn, Germany.
J WinterDepartment of Periodontology, Operative and Preventive Dentistry, University of Bonn, Welschnonnenstr. 17, 53111, Bonn, Germany. jochen.winter@ukb.uni-bonn.de.
University of Bonn · DEUniversity Hospital Bonn · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The impact of oral pathogens onto the generation and variability of oral tumors has only recently been investigated. To get further insights, oral cancer cells were treated with pathogens and additionally, as a result of this bacterial cellular infection, with human defensins, which are as anti-microbial peptide members of the innate immune system. After cell stimulation, proliferation behavior, expression analysis of oncogenic relevant defensin genes, and effects on EGFR signaling were investigated. The expression of oncogenic relevant anti-microbial peptides was analyzed with real-time PCR and immunohistochemistry. Cell culture experiments were performed to examine cellular impacts caused by stimulation, i.e., altered gene expression, proliferation rate, and EGF receptor-dependent signaling. Incubation of oral tumor cells with an oral pathogen (Porphyromonas gingivalis) and human α-defensins led to an increase in cell proliferation. In contrast, another oral bacterium used, Aggregatibacter actinomycetemcomitans, enhanced cell death. The bacteria and anti-microbial peptides exhibited diverse effects on the transcript levels of oncogenic relevant defensin genes and epidermal growth factor receptor signaling. These two oral pathogens exhibited opposite primary effects on the proliferation behavior of oral tumor cells. Nevertheless, both microbe species led to similar secondary impacts on the proliferation rate by modifying expression levels of oncogenic relevant α-defensin genes. In this respect, oral pathogens exerted multiplying effects on tumor cell proliferation. Additionally, human defensins were shown to differently influence epidermal growth factor receptor signaling, supporting the hypothesis that these anti-microbial peptides serve as ligands of EGFR, thus modifying the proliferation behavior of oral tumor cells.

Indexed as

Aggregatibacter actinomycetemcomitansAmino Acid SequenceAnti-Infective AgentsApoptosisBlotting, WesternCarcinoma, Squamous CellCase-Control StudiesCell ProliferationDefensinsFollow-Up StudiesGene Expression Regulation, NeoplasticGingivaHead and Neck NeoplasmsHumansImmunoenzyme TechniquesMouth NeoplasmsAnti-Infective AgentsDefensinsRNA, MessengerEGFR signalingHuman defensinsOSCCProliferation

Identifiers

PMID27481514
OpenAlexW2494118181

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.