ArticleJournal of microbiology (Seoul, Korea)2016
Mycobacterium tuberculosis gene expression at different stages of hypoxia-induced dormancy and upon resuscitation.
Article in Journal of microbiology (Seoul, Korea), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 55 citations in OpenAlex.
- Circulating antibody signatures againstFrontiers in immunology · 2026Article
- Exploring the Protein Landscape of Dormant Mycobacterium tuberculosis Through In Vitro Functional Studies.Advances in experimental medicine and biology · 2026Review
- Expression of Resuscitation-Promoting Factor C Stimulates the Growth ofInternational journal of microbiology · 2025Article
- Physiological stage-dependent effects ofFrontiers in microbiology · 2025Article
- Adaptation of the Mycobacterium tuberculosis transcriptome to biofilm growth.PLoS pathogens · 2024Article
- Microneedle patch-based enzyme-linked immunosorbent assay to quantify protein biomarkers of tuberculosis.Biomedical microdevices · 2024Article
- Regulatory role ofFrontiers in microbiology · 2024Article
- Identification of Rv1133c (MetE) as a marker ofFrontiers in immunology · 2024Article
- Eradication of Drug-TolerantMicroorganisms · 2023Review
- Article
- Intranasal multivalent adenoviral-vectored vaccine protects against replicating and dormant M.tb in conventional and humanized mice.NPJ vaccines · 2023Article
- Review
- Mycobacterium tuberculosis whiB3 and Lipid Metabolism Genes Are Regulated by Host Induced Oxidative StressMicroorganisms · 2022Article
- Optimal Detection of Latent Mycobacterium tuberculosis Infection by Combined Heparin-Binding Hemagglutinin (HBHA) and Early Secreted Antigenic Target 6 (ESAT-6) Whole-Blood Interferon Gamma Release Assays.Journal of clinical microbiology · 2022Article
- WhiB4 Is Required for the Reactivation of Persistent Infection of Mycobacterium marinum in Zebrafish.Microbiology spectrum · 2022Article
- Immuno-Diagnosis of Active Tuberculosis by a Combination of Cytokines/Chemokines Induced by Two Stage-Specific Mycobacterial Antigens: A Pilot Study in a Low TB Incidence Country.Frontiers in immunology · 2022Article
- Implications of drug-induced phenotypical resistance: Is isoniazid radicalizingFrontiers in antibiotics · 2022Article
- The Regulation of ManLAM-Related Gene Expression inInfection and drug resistance · 2022Article
- Article
- Tuberculosis: The success tale of less explored dormantFrontiers in cellular and infection microbiology · 2022Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The physiology of dormant Mycobacterium tuberculosis was studied in detail by examining the gene expression of 51 genes using quantitative Reverse-Transcription Polymerase Chain Reaction. A forty-day period of dormancy in the Wayne culture model depicted four major transcription patterns. Some sigma factors and many metabolic genes were constant, whereas genes belonging to the dormancy regulon were activated on day 9. In particular, alpha-crystallin mRNA showed more than a 1,000-fold increase compared to replicating bacilli. Genes belonging to the enduring hypoxic response were up-regulated at day 16, notably, transcription factors sigma B and E. Early genes typical of log-phase bacilli, esat-6 and fbpB, were uniformly down-regulated during dormancy. Late stages of dormancy showed a drop in gene expression likely due to a lack of substrates in anaerobic respiration as demonstrated by the transcriptional activation observed following nitrates addition. Among genes involved in nitrate metabolism, narG was strongly up-regulated by nitrates addition. Dormant bacilli responded very rapidly when exposed to oxygen and fresh medium, showing a transcriptional activation of many genes, including resuscitation-promoting factors, within one hour. Our observations extend the current knowledge on dormant M. tuberculosis gene expression and its response to nutrients and to aerobic and anaerobic respiration.
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