Evidence map›Paper›PMID 27480511›Full record

ArticleToxicology in vitro : an international journal published in association with BIBRA2016

Metabolomics evaluation of the impact of smokeless tobacco exposure on the oral bacterium Capnocytophaga sputigena.

Jinchun Sun, Jinshan Jin, Richard D Beger, Carl E Cerniglia, Maocheng Yang, Huizhong Chen

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Toxicology in vitro : an international journal published in association with BIBRA, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed, 1 pooled it
1.3field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 1 synthesis or guideline pooled it, 15 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Article
  4. Microbiology of the American Smokeless Tobacco.Applied microbiology and biotechnology · 2021
    Review
  5. Smoking and periodontal microorganisms.The Japanese dental science review · 2019
    Review
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Jinchun SunDivision of Systems Biology, National Center for Toxicological Research, US FDA, 3900 NCTR Rd, Jefferson, AR 72079, United States. Electronic address: Jinchun.Sun@fda.hhs.gov.
Jinshan JinDivision of Microbiology, National Center for Toxicological Research, US FDA, 3900 NCTR Rd, Jefferson, AR 72079, United States.
Richard D BegerDivision of Systems Biology, National Center for Toxicological Research, US FDA, 3900 NCTR Rd, Jefferson, AR 72079, United States.
Carl E CernigliaDivision of Microbiology, National Center for Toxicological Research, US FDA, 3900 NCTR Rd, Jefferson, AR 72079, United States.
Maocheng YangOffice of Science, Center for Tobacco Products, US FDA, 10903 New Hampshire Ave, Silver Spring, MD 20993, United States. Electronic address: Maocheng.Yang@fda.hhs.gov.
Huizhong ChenDivision of Microbiology, National Center for Toxicological Research, US FDA, 3900 NCTR Rd, Jefferson, AR 72079, United States. Electronic address: Huizhong.Chen@fda.hhs.gov.
National Center for Toxicological Research · USUnited States Food and Drug Administration · US

Funding

Intramural FDA HHS FD999999
6 · The paper itself

Abstract

The association between exposure to smokeless tobacco products (STP) and oral diseases is partially due to the physiological and pathological changes in the composition of the oral microbiome and its metabolic profile. However, it is not clear how STPs affect the physiology and ecology of oral microbiota. A UPLC/QTof-MS-based metabolomics study was employed to analyze metabolic alterations in oral bacterium, Capnocytophaga sputigena as a result of smokeless tobacco exposure and to assess the capability of the bacterium to metabolize nicotine. Pathway analysis of the metabolome profiles indicated that smokeless tobacco extracts caused oxidative stress in the bacterium. The metabolomics data also showed that the arginine-nitric oxide pathway was perturbed by the smokeless tobacco treatment. Results also showed that LC/MS was useful in identifying STP constituents and additives, including caffeine and many flavoring compounds. No significant changes in levels of nicotine and its major metabolites were found when C. sputigena was cultured in a nutrient rich medium, although hydroxylnicotine and cotinine N-oxide were detected in the bacterial metabolites suggesting that nicotine metabolism might be present as a minor degradation pathway in the bacterium. Study results provide new insights regarding the physiological and toxicological effects of smokeless tobacco on oral bacterium C. sputigena and associated oral health as well as measuring the ability of the oral bacterium to metabolize nicotine.

Indexed as

CapnocytophagaMetabolomicsNicotineOxidative StressTobacco, SmokelessNicotineMetabolomicsOral bacteriaSmokeless tobaccoToxicology

Identifiers

PMID27480511
PMCPMC5683397
OpenAlexW2490057276

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.