ReviewHistochemistry and cell biology2016
Immunohistochemical techniques for the human inner ear.
Review in Histochemistry and cell biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 45 citations in OpenAlex.
- Integrated Histology and Molecular Profiling of Postmortem Human Auditory and Vestibular Organs via a Poly (Methyl Methacrylate)-Based Workflow.Journal of the Association for Research in Otolaryngology : JARO · 2026Article
- Expression of Calcitonin Gene-related Peptide and Its Application to Cochlear Implantation Under Local Anesthesia With Monitored Anesthesia Care in the Very Elderly: The University of California, Los Angeles, Experience.Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology · 2026Article
- Changes in the Neurovascular Unit in Meniere's Disease.Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology · 2025Article
- A Case of Calcification Discovered Within the Membranous Labyrinth During Resection of a Vestibular Schwannoma.Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology · 2025Article
- Blood Labyrinth Barrier Waste Clearance as Assessed by GBCAs on MRI: A Scoping Review.Laryngoscope investigative otolaryngology · 2025Review
- Development of a non-human primate model for preclinical research of a novel auditory nerve implant.Frontiers in neuroscience · 2025Article
- Single-cell transcriptomic atlas reveals increased regeneration in diseased human inner ear balance organs.Nature communications · 2024Article
- Dynamic Molecular Markers of Otosclerosis in the Human Cochlea.The Annals of otology, rhinology, and laryngology · 2024Article
- Immunohistochemistry Reveals TRPC Channels in the Human Hearing Organ-A Novel CT-Guided Approach to the Cochlea.International journal of molecular sciences · 2023Article
- Immunohistochemical localization of glucocorticoid receptors in the human cochlea.Brain research · 2023Article
- Mortui vivos docent: a modern revival of temporal bone plug harvests.Frontiers in neuroscience · 2023Article
- Mutation of SLC7A14 causes auditory neuropathy and retinitis pigmentosa mediated by lysosomal dysfunction.Science advances · 2022Article
- Identification of a genetic variant underlying familial cases of recurrent benign paroxysmal positional vertigo.PloS one · 2021Article
- Immune Response of Macrophage Population to Cochlear Implantation: Cochlea Immune Cells.Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology · 2020Article
- Comparison of ethylenediaminetetraacetic acid and rapid decalcificier solution for studying human temporal bones by immunofluorescence.Laryngoscope investigative otolaryngology · 2020Article
- Mouse Models of Human Pathogenic Variants ofGenes · 2020Article
- NEDDylation negatively regulates ERRβ expression to promote breast cancer tumorigenesis and progression.Cell death & disease · 2020Article
- Histology of the Cochlear Outer Sulcus Cells in Normal Human Ears, Presbycusis, and Menière's Disease.Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology · 2020Article
- Experimental Animal Models for Meniere's Disease: A Mini-Review.Journal of audiology & otology · 2020Review
- Characterizing Adult Cochlear Supporting Cell Transcriptional Diversity Using Single-Cell RNA-Seq: Validation in the Adult Mouse and Translational Implications for the Adult Human Cochlea.Frontiers in molecular neuroscience · 2020Article
Corrections and comments
- Erratum issued
Authors and funding
7 authors at 1 institution in 2 countries.
Funding
Abstract
In this review, we provide a description of the recent methods used for immunohistochemical staining of the human inner ear using formalin-fixed frozen, paraffin and celloidin-embedded sections. We also show the application of these immunohistochemical methods in auditory and vestibular endorgans microdissected from the human temporal bone. We compare the advantages and disadvantages of immunohistochemistry (IHC) in the different types of embedding media. IHC in frozen and paraffin-embedded sections yields a robust immunoreactive signal. Both frozen and paraffin sections would be the best alternative in the case where celloidin-embedding technique is not available. IHC in whole endorgans yields excellent results and can be used when desiring to detect regional variations of protein expression in the sensory epithelia. One advantage of microdissection is that the tissue is processed immediately and IHC can be made within 1 week of temporal bone collection. A second advantage of microdissection is the excellent preservation of both morphology and antigenicity. Using celloidin-embedded inner ear sections, we were able to detect several antigens by IHC and immunofluorescence using antigen retrieval methods. These techniques, previously applied only in animal models, allow for the study of numerous important proteins expressed in the human temporal bone potentially opening up a new field for future human inner ear research.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.