ArticleMolecular biotechnology2016
Genetic Transformation of Hordeum vulgare ssp. spontaneum for the Development of a Transposon-Based Insertional Mutagenesis System.
Article in Molecular biotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- A Protocol for High-efficiency Transformation and Genome Editing in Elite Wheat Cultivars.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Metabolic engineering-induced transcriptome reprogramming of lipid biosynthesis enhances oil composition in oat.Plant biotechnology journal · 2024Article
- Transposable elements: multifunctional players in the plant genome.Frontiers in plant science · 2023Review
- Highly Efficient and Reproducible Genetic Transformation in Pea for Targeted Trait Improvement.ACS agricultural science & technology · 2022Article
- Toward the development of Ac/Ds transposon-mediated gene tagging system for functional genomics in oat (Avena sativa L.).Functional & integrative genomics · 2022Article
- Progress and challenges in sorghum biotechnology, a multipurpose feedstock for the bioeconomy.Journal of experimental botany · 2022Review
Corrections and comments
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Authors and funding
3 authors.
Funding
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Abstract
Domestication and intensive selective breeding of plants has triggered erosion of genetic diversity of important stress-related alleles. Researchers highlight the potential of using wild accessions as a gene source for improvement of cereals such as barley, which has major economic and social importance worldwide. Previously, we have successfully introduced the maize Ac/Ds transposon system for gene identification in cultivated barley. The objective of current research was to investigate the response of Hordeum vulgare ssp. spontaneum wild barley accessions in tissue culture to standardize parameters for introduction of Ac/Ds transposons through genetic transformation. We investigated the response of ten wild barley genotypes for callus induction, regenerative green callus induction and regeneration of fertile plants. The activity of exogenous Ac/Ds elements was observed through a transient assay on immature wild barley embryos/callus whereby transformed embryos/calli were identified by the expression of GUS. Transient Ds expression bombardment experiments were performed on 352 pieces of callus (3-5 mm each) or immature embryos in 4 genotypes of wild barley. The transformation frequency of putative transgenic callus lines based on transient GUS expression ranged between 72 and100 % in wild barley genotypes. This is the first report of a transformation system in H. vulgare ssp. spontaneum.
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Registered trials
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