Evidence map›Paper›PMID 27479354›Full record

ArticleScientific data2016

Serial femtosecond crystallography datasets from G protein-coupled receptors.

Thomas A White, Anton Barty, Wei Liu, Andrii Ishchenko, Haitao Zhang, Cornelius Gati, Nadia A Zatsepin, Shibom Basu, Dominik Oberthür, Markus Metz and 10 more

Open access · goldAbstract readComment
In one paragraph

Article in Scientific data, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.0field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 13 citations in OpenAlex.

  1. Detector alignment for X-ray crystallography usingJournal of applied crystallography · 2026
    Article
  2. Article
  3. Article
  4. 3D-printed holders forJournal of applied crystallography · 2020
    Article
  5. Journal of applied crystallography · 2019
    Article
  6. Processing serial crystallography data with CrystFEL: a step-by-step guide.Acta crystallographica. Section D, Structural biology · 2019
    Article
  7. IUCrJ · 2019
    Article
  8. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

20 authors at 8 institutions in 5 countries.

Thomas A WhiteCenter for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestraße 85, 22607 Hamburg, Germany.
Anton BartyCenter for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestraße 85, 22607 Hamburg, Germany.
Wei LiuSchool of Molecular Sciences, and Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, Arizona 85287-1604, USA.
Andrii IshchenkoDepartments of Chemistry and Physics &Astronomy, The Bridge Institute, University of Southern California, Los Angeles, California 90089, USA.
Haitao ZhangDepartments of Chemistry and Physics &Astronomy, The Bridge Institute, University of Southern California, Los Angeles, California 90089, USA.
Cornelius GatiMRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge CB2 0QH, UK.
Nadia A ZatsepinDepartment of Physics, Arizona State University, Tempe, Arizona 85287, USA.
Shibom BasuSchool of Molecular Sciences, and Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, Arizona 85287-1604, USA.
Dominik OberthürCenter for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestraße 85, 22607 Hamburg, Germany.
Markus MetzCenter for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestraße 85, 22607 Hamburg, Germany.
Kenneth R BeyerleinCenter for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestraße 85, 22607 Hamburg, Germany.
Chun Hong YoonCenter for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestraße 85, 22607 Hamburg, Germany.
Oleksandr M YefanovCenter for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestraße 85, 22607 Hamburg, Germany.
Daniel JamesPaul Scherrer Institute, Villigen CH-5232, Switzerland.ORCID http://orcid.org/0000-0002-8348-6661
Dingjie WangiHuman Institute, ShanghaiTech University, 2F Building 6, 99 Haike Road, Pudong New District, Shanghai 201210, China.
Marc MesserschmidtNational Science Foundation BioXFEL Science and Technology Center, 700 Ellicott Street, Buffalo, New York 14203, USA.
Jason E KoglinLinac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA.
Sébastien BoutetLinac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA.
Uwe WeierstallDepartment of Physics, Arizona State University, Tempe, Arizona 85287, USA.
Vadim CherezovDepartments of Chemistry and Physics &Astronomy, The Bridge Institute, University of Southern California, Los Angeles, California 90089, USA.ORCID http://orcid.org/0000-0002-5265-3914
Universität Hamburg · DEArizona State University · USUniversity of Southern California · USSLAC National Accelerator Laboratory · USMRC Laboratory of Molecular Biology · GBPaul Scherrer Institute · CHShanghaiTech University · CNU.S. National Science Foundation · US

Funding

Project 3U54GM094618 · NIGMS · UNIVERSITY OF SOUTHERN CALIFORNIA · PI STEVENS, RAYMOND C · 2010 to 2014
$17.1M
Structure-Function of Opioid ReceptorsP01DA035764 · NIDA · UNIVERSITY OF SOUTHERN CALIFORNIA · PI CHEREZOV, VADIM, ROTH, BRYAN L. · 2014 to 2018
$7.1M
HT structure determination of GPCRs by LCP serial femtosecond nanocrystallographyR01GM108635 · NIGMS · UNIVERSITY OF SOUTHERN CALIFORNIA · PI CHEREZOV, VADIM · 2014 to 2017
$1.5M
Medical Research Council MC_UP_A025_1013NIDA NIH HHS P01 DA035764NIGMS NIH HHS R01 GM108635NIGMS NIH HHS U54 GM094618
6 · The paper itself

Abstract

We describe the deposition of four datasets consisting of X-ray diffraction images acquired using serial femtosecond crystallography experiments on microcrystals of human G protein-coupled receptors, grown and delivered in lipidic cubic phase, at the Linac Coherent Light Source. The receptors are: the human serotonin receptor 2B in complex with an agonist ergotamine, the human δ-opioid receptor in complex with a bi-functional peptide ligand DIPP-NH2, the human smoothened receptor in complex with an antagonist cyclopamine, and finally the human angiotensin II type 1 receptor in complex with the selective antagonist ZD7155. All four datasets have been deposited, with minimal processing, in an HDF5-based file format, which can be used directly for crystallographic processing with CrystFEL or other software. We have provided processing scripts and supporting files for recent versions of CrystFEL, which can be used to validate the data.

Indexed as

CrystallographyLasersHumansLipidsSoftwareX-Ray DiffractionLipids

Identifiers

PMID27479354
PMCPMC4968193
OpenAlexW2481904852

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.