ArticleTransplantation2016
Eight-Hour Continuous Normothermic Ex Vivo Kidney Perfusion Is a Safe Preservation Technique for Kidney Transplantation: A New Opportunity for the Storage, Assessment, and Repair of Kidney Grafts.
Article in Transplantation, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 1 of them a synthesis that pooled it.
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Who cites it
31 citing papers in PubMed, 1 synthesis or guideline pooled it, 66 citations in OpenAlex.
- Perfusate Composition and Duration ofTransplant international : official journal of the European Society for Organ Transplantation · 2022Pooled it
- Metabolomic Insight into Donation After Circulatory-Death Kidney Grafts in Porcine Autotransplant Model: Normothermic Ex Vivo Kidney Perfusion Compared with Hypothermic Machine Perfusion and Static Cold Storage.International journal of molecular sciences · 2025Article
- Deceased donor kidney function and branched chain amino acid metabolism during ex vivo normothermic perfusion.Kidney international · 2024Article
- Normothermic ex vivo kidney perfusion preserves mitochondrial and graft function after warm ischemia and is further enhanced by AP39.Nature communications · 2024Article
- Subnormothermic Oxygenated Machine Perfusion (24 h) in DCD Kidney Transplantation.Transplantation direct · 2024Article
- A machine-learning approach to human ex vivo lung perfusion predicts transplantation outcomes and promotes organ utilization.Nature communications · 2023Article
- The Sigma-1 Receptor Is a Novel Target for Improving Cold Preservation in Rodent Kidney Transplants.International journal of molecular sciences · 2023Article
- Expanding the Horizons of Pre-Transplant Renal Vascular Assessment Using Ex Vivo Perfusion.Current issues in molecular biology · 2023Review
- The impact of oxygen supply and erythrocytes during normothermic kidney perfusion.Scientific reports · 2023Article
- External pressure dynamics promote kidney viability and perfusate filtration during ex vivo kidney perfusion.Scientific reports · 2022Article
- Sense and Sensibilities of Organ Perfusion as a Kidney and Liver Viability Assessment Platform.Transplant international : official journal of the European Society for Organ Transplantation · 2022Review
- Prolonged warm ischemia time leads to severe renal dysfunction of donation-after-cardiac death kidney grafts.Scientific reports · 2021Article
- Recent Approaches to Targeting Canonical NFJournal of the American Society of Nephrology : JASN · 2021Review
- Urine recirculation prolongs normothermic kidney perfusion via more optimal metabolic homeostasis-a proteomics study.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons · 2021Article
- Normothermic Ex-vivo Kidney Perfusion in a Porcine Auto-Transplantation Model Preserves the Expression of Key Mitochondrial Proteins: An Unbiased Proteomics Analysis.Molecular & cellular proteomics : MCP · 2021Article
- Review
- [Machine perfusion for conditioning liver and kidneys before transplantation].Der Chirurg; Zeitschrift fur alle Gebiete der operativen Medizen · 2020Review
- Kidney Perfusion as an Organ Quality Assessment Tool-Are We Counting Our Chickens Before They Have Hatched?Journal of clinical medicine · 2020Review
- The future of organ perfusion and re-conditioning.Transplant international : official journal of the European Society for Organ Transplantation · 2019Review
- [Modern concepts for the dynamic preservation of the liver and kidneys in the context of transplantation].Der Pathologe · 2019Review
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Authors and funding
16 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHypothermic kidney storage causes preservation injury and is poorly tolerated by renal grafts. We investigated whether static cold storage (SCS) can be safely replaced with a novel technique of pressure-controlled normothermic ex vivo kidney perfusion (NEVKP) in heart-beating donor kidney transplantation.
methodsRight kidneys were removed from 30 kg Yorkshire pigs in a model of heart-beating donation and either preserved in cold histidine-tryptophan-ketoglutarate solution for 8 hours (n = 5), or subjected to 8 hours of pressure-controlled NEVKP (n = 5) followed by renal heterotopic autotransplantation.
resultsDuring NEVKP, physiologic perfusion conditions were maintained with low intrarenal resistance and normal electrolyte and pH parameters. Aspartate aminotransferase and lactate dehydrogenase as injury markers were below the detectable analyzer range (<4 and <100 U/L, respectively). Perfusate lactate concentration decreased from baseline until the end of perfusion (10.38 ± 0.76 mmol/L vs 1.22 ± 0.26 mmol/L; P < 0.001). Posttransplantation, animals transplanted with NEVKP versus SCS grafts demonstrated similar serum creatinine peak levels (NEVKP, 2.0 ± 0.5 vs SCS 2.7 ± 0.7 mg/dL; P = 0.11) and creatinine clearance on day 10 (NEVKP, 65.9 ± 18.8 mL/min vs SCS 61.2 ± 15.6 mL/min; P = 0.74). After 10 days of follow-up, animals transplanted with NEVKP grafts had serum creatinine and blood urea nitrogen values comparable to their basal levels (P = 0.49 and P = 0.59), whereas animals transplanted with SCS grafts had persistently elevated serum creatinine and blood urea nitrogen when compared with basal levels (P = 0.01 and P = 0.03).
conclusionsContinuous pressure-controlled NEVKP is feasible and safe in good quality heart-beating donor kidney grafts. It maintains a physiologic environment and excellent graft function ex vivo during preservation without causing graft injury.
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