ArticleBiomicrofluidics2016
Rheology of clustering protein solutions.
Article in Biomicrofluidics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed.
- Article
- Microfluidic techniques for mechanical measurements of biological samples.Biophysics reviews · 2023Review
- Protein Adhesives: Investigation of Factors Affecting Wet Strength of Alkaline Treated Proteins Crosslinked with Glyoxal.Polymers · 2022Article
- A small-volume microcapillary rheometer.Rheologica acta · 2022Article
- A microfluidic approach to studying the injection flow of concentrated albumin solutions.SN applied sciences · 2021Article
- Experimental Evidence for a Cluster Glass Transition in Concentrated Lysozyme Solutions.The journal of physical chemistry. B · 2019Article
- Colloidal systems with a short-range attraction and long-range repulsion: Phase diagrams, structures, and dynamics.Current opinion in colloid & interface science · 2019Article
- Improving Viscosity and Stability of a Highly Concentrated Monoclonal Antibody Solution with Concentrated Proline.Pharmaceutical research · 2018Article
- The NISTmAb Reference Material 8671 lifecycle management and quality plan.Analytical and bioanalytical chemistry · 2018Article
- Jetting of a shear banding fluid in rectangular ducts.Physical review fluids · 2017Article
- Preface to Special Topic: Invited Articles on Microfluidic Rheology.Biomicrofluidics · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High viscosity is a major challenge with protein therapeutics at extremely high concentrations. To overcome this obstacle, it is essential to understand the relationship between the concentration of a protein solution and its viscosity as a function of shear rate and temperature. Here, lysozyme is a model charged globular protein having both short-ranged attraction (SA) and long-ranged repulsion (LR) that promote the formation of dynamic clusters at high concentrations. We report viscosity measurements from a micro-capillary rheometer (using only several microliters of solution) over a wide range of lysozyme solution concentrations, shear rates, and temperatures. Solution structural relaxation dynamics are also probed by dynamic light scattering (DLS). As a result of lysozyme's SALR interactions, the viscosity increased dramatically across all shear rates with increasing concentration and decreasing temperature. While most of the solutions exhibited Newtonian behavior, shear thinning was exhibited at the highest concentration (480 g/l) and lowest temperatures at shear rates above approximately 10(4 )s(-1). The onset shear rate for thinning and a structural relaxation rate estimated from a slow-mode measured by DLS are compared. These measurements provide insights into the properties of protein solutions and their microscopic structural origins.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.