ArticleNutrition & metabolism2016
Exposure to dietary lipid leads to rapid production of cytosolic lipid droplets near the brush border membrane.
Article in Nutrition & metabolism, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 25 citations in OpenAlex.
- A single bout of resistance exercise improves postprandial lipid metabolism in overweight/obese men with prediabetes.Diabetologia · 2020Trial
- Vitamin E absorption and kinetics in healthy women, as modulated by food and by fat, studied using 2 deuterium-labeled α-tocopherols in a 3-phase crossover design.The American journal of clinical nutrition · 2019Trial
- Lipid droplets in central nervous system and functional profiles of brain cells containing lipid droplets in various diseases.Journal of neuroinflammation · 2025Review
- Interplay between lipid droplets and alpha-synuclein: implication in Parkinson's disease pathogenesis.Frontiers in molecular neuroscience · 2025Review
- Isolation and Lipidomic Profiling of Neuronal Lipid Droplets: Unveiling the Lipid Landscape for insights into Neurodegenerative Disorders.bioRxiv : the preprint server for biology · 2023Article
- Reduced circulating FABP2 in patients with moderate to severe COVID-19 may indicate enterocyte functional change rather than cell death.Scientific reports · 2022Article
- Lipid droplets in the nervous system.The Journal of cell biology · 2021Review
- Jejunal villus absorption and paracellular tight junction permeability are major routes for early intestinal uptake of food-grade TiOParticle and fibre toxicology · 2020Article
- Regulation of intestinal lipid metabolism: current concepts and relevance to disease.Nature reviews. Gastroenterology & hepatology · 2020Review
- Intestinal basolateral lipid substrate transport is linked to chylomicron secretion and is regulated by apoC-III.Journal of lipid research · 2019Article
- Cholesterol trafficking and raft-like membrane domain composition mediate scavenger receptor class B type 1-dependent lipid sensing in intestinal epithelial cells.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2018Article
- FABP1 knockdown in human enterocytes impairs proliferation and alters lipid metabolism.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2017Article
Corrections and comments
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Authors and funding
17 authors at 8 institutions in 3 countries.
Funding
Abstract
backgroundIntestinal absorption of dietary lipids involves their hydrolysis in the lumen of proximal intestine as well as uptake, intracellular transport and re-assembly of hydrolyzed lipids in enterocytes, leading to the formation and secretion of the lipoproteins chylomicrons and HDL. In this study, we examined the potential involvement of cytosolic lipid droplets (CLD) whose function in the process of lipid absorption is poorly understood.
methodsIntestinal lipid absorption was studied in mouse after gavage. Three populations of CLD were purified by density ultracentrifugations, as well as the brush border membranes, which were analyzed by western-blots. Immunofluorescent localization of membranes transporters or metabolic enzymes, as well as kinetics of CLD production, were also studied in intestine or Caco-2 cells.
resultsWe isolated three populations of CLD (ranging from 15 to 1000 nm) which showed differential expression of the major lipid transporters scavenger receptor BI (SR-BI), cluster of differentiation 36 (CD-36), Niemann Pick C-like 1 (NPC1L1), and the ATP-binding cassette transporters ABCG5/G8 but also caveolin 2 and fatty acid binding proteins. The enzyme monoacylglycerol acyltransferase 2 (MGAT2) was identified in the brush border membrane (BBM) in addition to the endoplasmic reticulum, suggesting local synthesis of triglycerides and CLD at both places.
conclusionsWe show a very fast production of CLD by enterocytes associated with a transfer of apical constituents as lipid transporters. Our findings suggest that following their uptake by enterocytes, lipids can be partially metabolized at the BBM and packaged into CLD for their transportation to the ER.
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