ArticleEngineering in life sciences2016
Protein adsorption onto nanoparticles induces conformational changes: Particle size dependency, kinetics, and mechanisms.
Article in Engineering in life sciences, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
46 citing papers in PubMed, 1 synthesis or guideline pooled it, 172 citations in OpenAlex.
- Meta-Analysis and Machine Learning Prediction of Protein Corona Composition across Nanoparticle Systems in Biological Media.ACS nano · 2025Pooled it
- The protein corona at the nano-bio interface: the need for standardized methodology and opportunities for neurodegenerative disease intervention.RSC advances · 2026Review
- Engineering Biomimetic Nanoparticle Performance Through Fabrication Method Selection: Turbulent Jet Mixing, Microfluidics, and Extrusion.Small methods · 2026Article
- Residue-Specific Dock-Loosen-Unfold Mechanism of GB1 on Nanoparticle Surfaces Revealed by Kinetic and Φ-Value Analysis.Biomolecules · 2026Article
- Plastic-binding peptides as anchors for protein scaffolds on synthetic plastics: opportunities and challenges.RSC advances · 2025Article
- Quantitative Analysis of Virus Adsorption and Co-adsorption Behavior Using BET Modeling and SERS Spectroscopy.The journal of physical chemistry. A · 2025Article
- Mechanistic Modeling of Size and Surface Charge Changes of Gold and Silver Nanoparticles during In Vitro Nanoparticle Protein Corona Formation.ACS pharmacology & translational science · 2025Article
- Evaluating Biocompatibility: From Classical Techniques to State-of-the-Art Functional Proteomics.Nanomaterials (Basel, Switzerland) · 2025Review
- Protein Adsorption on Nano- and Microparticles: Dependence on Morphological and Physicochemical Properties of Particles and Effect on Particle-Cell Interactions.Nanomaterials (Basel, Switzerland) · 2025Review
- Impact of Milk on the Behavior and Toxicity of Nanosized SiOACS omega · 2025Article
- Autonomous Abiotic Thermal Protectant for Immunoglobulin G: Reducing the Need for Cold Chain Storage.Biomacromolecules · 2025Article
- The Protein Corona Paradox: Challenges in Achieving True Biomimetics in Nanomedicines.Biomimetics (Basel, Switzerland) · 2025Review
- Effects of buffers on spray-freeze-dried/lyophilized high concentration protein formulations.International journal of pharmaceutics · 2025Article
- l-Type amino acid transporter 1-targeting nanoparticles for antisense oligonucleotide delivery to the CNS.Molecular therapy. Nucleic acids · 2024Article
- Label free tracking to quantify nanoparticle diffusion through biological media.Scientific reports · 2024Article
- A comprehensive investigation of the interactions of human serum albumin with polymeric and hybrid nanoparticles.Drug delivery and translational research · 2024Article
- Interaction of Serum and Plasma Proteins with Polyelectrolyte Microparticles with Core/Shell and Shell-Only Structures.ACS omega · 2024Article
- Bovine Serum Albumin Bends Over Backward to Interact with Aged Plastics: A Model for Understanding Protein Attachment to Plastic Debris.Environmental science & technology · 2024Article
- A Review for Uncovering the "Protein-Nanoparticle Alliance": Implications of the Protein Corona for Biomedical Applications.Nanomaterials (Basel, Switzerland) · 2024Review
- Plant-mediated green synthesis of gold nanoparticles using an aqueous extract ofSaudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society · 2024Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
The use of nanomaterials in bioapplications demands a detailed understanding of protein-nanoparticle interactions. Proteins can undergo conformational changes while adsorbing onto nanoparticles, but studies on the impact of particle size on conformational changes are scarce. We have shown that conformational changes happening upon adsorption of myoglobin and BSA are dependent on the size of the nanoparticle they are adsorbing to. Out of eight initially investigated model proteins, two (BSA and myoglobin) showed conformational changes, and in both cases this conformational change was dependent on the size of the nanoparticle. Nanoparticle sizes ranged from 30 to 1000 nm and, in contrast to previous studies, we attempted to use a continuous progression of sizes in the range found in live viruses, which is an interesting size of nanoparticles for the potential use as drug delivery vehicles. Conformational changes were only visible for particles of 200 nm and bigger. Using an optimized circular dichroism protocol allowed us to follow this conformational change with regard to the nanoparticle size and, thanks to the excellent temporal resolution also in time. We uncovered significant differences between the unfolding kinetics of myoglobin and BSA. In this study, we also evaluated the plausibility of commonly used explanations for the phenomenon of nanoparticle size-dependent conformational change. Currently proposed mechanisms are mostly based on studies done with relatively small particles, and fall short in explaining the behavior seen in our studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.