ArticleJournal of virological methods2016
Species-specific differentiation of variola, monkeypox, and varicella-zoster viruses by multiplex real-time PCR assay.
Article in Journal of virological methods, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers, 1 of them a synthesis that pooled it.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
63 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Guidelines for the Laboratory Diagnosis of Monkeypox in Korea.Annals of laboratory medicine · 2023Guideline
- Tecovirimat treatment of clade II mpox: a virological analysis of a randomised, double-blind, phase 3 trial.The Lancet. Microbe · 2026Trial
- Evaluation of a portable multiplex real-time pcr assay for the detection of monkeypox virus and non-variola orthopoxviruses.Virology journal · 2026Article
- A Global Health Alert on the Re-Emergence of Mpox a Viral Zoonotic Illness Its Prevention and Management: A Narrative Review.Health science reports · 2026Article
- Portable Detection of Monkeypox virus Clades using Bridging LAMP.Research square · 2026Article
- Evaluation and Clinical Validation of Pan-Specific and Clade-Specific Diagnostic Real-Time PCR Assays for Monkeypox Virus.Journal of medical virology · 2026Article
- Development of in-house PCR assays and cross-sectional molecular surveillance of infectious pathogens in adverse pregnancy outcomes and neonatal sepsis in central India.BMC infectious diseases · 2026Article
- Efficient whole-genome sequencing of Monkeypox virus using a novel nuclease-multiple displacement amplification enrichment method.Npj viruses · 2026Article
- Development and evaluation of a two-tube multiplex real-time PCR assay for differential detection of Monkeypox virus clades Ia, Ib, IIa, and IIb.Frontiers in microbiology · 2026Article
- A viral-vectored multipathogen vaccine platform based on replication-competent vaccinia virus and adeno-associated virus.Frontiers in immunology · 2026Article
- Patterns of genomic deletions in monkeypox virus during the 2022 outbreak in the United States.Nature communications · 2025Article
- A targeted tiled amplicon sequencing approach for clade and subclade level differentiation of monkeypox virus from wastewater.Scientific reports · 2025Article
- Development and Evaluation of a Molecular Test for Monkeypox Virus in the Federal District, Brazil.Genes · 2025Article
- Mpox unveiled: Global epidemiology, treatment advances, and prevention strategies.One health (Amsterdam, Netherlands) · 2025Review
- Orthopoxvirus Genome Sequencing, Assembly, and Analysis.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Mpox virus: virology, molecular epidemiology, and global public health challenges.Frontiers in microbiology · 2025Review
- Development of a two-tube multiplex real-time fluorescent PCR for the simultaneous differentiation of the mpox virus clades and the A.1, B.1 and C.1 lineages within clade IIb.Frontiers in cellular and infection microbiology · 2025Article
- Evaluation of analytical performance of the STANDARDEmerging microbes & infections · 2024Article
- CRISPR-based strategies for sample-to-answer monkeypox detection: current status and emerging opportunities.Nanotechnology · 2024Review
- Article
3 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A method of one-stage rapid detection and differentiation of epidemiologically important variola virus (VARV), monkeypox virus (MPXV), and varicella-zoster virus (VZV) utilizing multiplex real-time TaqMan PCR assay was developed. Four hybridization probes with various fluorescent dyes and the corresponding fluorescence quenchers were simultaneously used for the assay. The hybridization probes specific for the VARV sequence contained FAM/BHQ1 as a dye/quencher pair; MPXV-specific, JOE/BHQ1; VZV-specific, TAMRA/BHQ2; and internal control-specific, Cy5/BHQ3. The specificity and sensitivity of the developed method were assessed by analyzing DNA of 32 strains belonging to orthopoxvirus and herpesvirus species.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.