ArticleJournal of oral microbiology2016
A novel compound to maintain a healthy oral plaque ecology in vitro.
Article in Journal of oral microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 25 citations in OpenAlex.
- Case Report: Murine typhus complicated by symmetrical peripheral gangrene: first report and diagnostic insights from metagenomic next-generation sequencing.Frontiers in immunology · 2025Article
- Evaluating the Effectiveness ofPharmaceuticals (Basel, Switzerland) · 2024Article
- Silent signals: howFrontiers in oral health · 2024Review
- The effect of sex steroid hormones on the ecology ofBiofilm · 2023Article
- In Vitro Dental Plaque Culture Model for Biofilm Structural Analyses.Current protocols · 2023Article
- New Preventive Strategy against Oral Biofilm Formation in Caries-Active Children: An In Vitro Study.Antibiotics (Basel, Switzerland) · 2023Article
- Gram-negative quorum sensing signalling enhances biofilm formation and virulence traits in gram-positive pathogenJournal of oral microbiology · 2023Article
- Metataxonomic and metabolomic evidence of biofilm homeostasis disruption related to caries: An in vitro study.Molecular oral microbiology · 2022Article
- Plant Extract-Synthesized Silver Nanoparticles for Application in Dental Therapy.Pharmaceutics · 2022Review
- Acyl homoserine lactone-mediated quorum sensing in the oral cavity: a paradigm revisited.Scientific reports · 2020Article
- Short-ChainApplied and environmental microbiology · 2020Article
- Correlation between relative bacterial activity and lactate dehydrogenase gene expression of co-cultures in vitro.Clinical oral investigations · 2019Article
- Short-Time Antibacterial Effects of Dimethylaminododecyl Methacrylate on Oral Multispecies Biofilm In Vitro.BioMed research international · 2019Article
- Beyond Streptococcus mutans: clinical implications of the evolving dental caries aetiological paradigms and its associated microbiome.British dental journal · 2018Review
- Saliva-Derived Commensal and Pathogenic Biofilms in a Human Gingiva Model.Journal of dental research · 2018Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveDental caries is caused by prolonged episodes of low pH due to acid production by oral biofilms. Bacteria within such biofilms communicate via quorum sensing (QS). QS regulates several phenotypic biofilm parameters, such as biofilm formation and the production of virulence factors. In this study, we evaluated the effect of several QS modifiers on growth and the cariogenic potential of microcosm oral biofilms.
methodsBiofilms were inoculated with pooled saliva and cultured in the presence of sucrose for 48 and 96 h. QS modifiers (or carrier controls) were continuously present. Lactic acid accumulation capacities were compared to evaluate the cariogenic potential of the biofilms. Subsequently, biofilm growth was quantified by determining colony forming unit counts (CFUs) and their ecology by 16S rDNA-based microbiome analyses. The minimal inhibitory concentration (MIC) for several Streptococcus spp. was determined using microbroth dilution.
resultsOf the tested QS modifiers only 3-oxo-N-(2-oxocyclohexyl)dodecanamide (3-Oxo-N) completely abolished lactic acid accumulation by the biofilms without affecting biofilm growth. This compound was selected for further investigation. The active range of 3-Oxo-N was 10-100 µM. The homologous QS molecule, acyl homoserine lactone C12, did not counteract the reduction in lactic acid accumulation, suggesting a mechanism other than QS inhibition. Microbial ecology analyses showed a reduction in the relative abundance of Streptococcus spp. in favor of the relative abundance of Veillonella spp. in the 3-Oxo-N exposed biofilms. The MIC of 3-Oxo-N for several streptococcal species varied between 8 and 32 µM.
conclusion3-Oxo-N changes the ecological homeostasis of in vitro dental plaque. It reduces its cariogenic potential by minimizing lactic acid accumulation. Based on our in vitro data, 3-Oxo-N represents a promising compound in maintaining a healthy, non-cariogenic, ecology in in vivo dental plaque.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.