ArticleMolecular and cellular biology1989
Identification and developmental expression of a smooth-muscle gamma-actin in postmeiotic male germ cells of mice.
Article in Molecular and cellular biology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 64 citations in OpenAlex.
- Article
- Identification and validation of mouse sperm proteins correlated with epididymal maturation.Proteomics · 2011Article
- Sperm length is not influenced by haploid gene expression in the flies Drosophila melanogaster and Scathophaga stercoraria.Proceedings. Biological sciences · 2009Article
- Separation of spermatogenic cells from adult transgenic mouse testes using unit-gravity sedimentation.Molecular biotechnology · 2003Article
- Transforming growth factor-beta induction of smooth muscle cell phenotpye requires transcriptional and post-transcriptional control of serum response factor.The Journal of biological chemistry · 2002Article
- Telokin expression is restricted to smooth muscle tissues during mouse development.American journal of physiology. Cell physiology · 2001Article
- Alterations in the expression of the beta-cytoplasmic and the gamma-smooth muscle actins in hypertrophied urinary bladder smooth muscle.Molecular and cellular biochemistry · 1994Article
- Binding of a phosphoprotein to the 3' untranslated region of the mouse protamine 2 mRNA temporally represses its translation.Molecular and cellular biology · 1993Article
- Analysis of the mouse protamine 1 promoter in transgenic mice.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
- Structure, chromosome location, and expression of the human smooth muscle (enteric type) gamma-actin gene: evolution of six human actin genes.Molecular and cellular biology · 1991Article
- List of cloned mouse genes with unique expression patterns during spermatogenesis.Mammalian genome : official journal of the International Mammalian Genome Society · 1991Article
- Mouse map of paralogous genes.Mammalian genome : official journal of the International Mammalian Genome Society · 1991Review
- The nucleotide sequence of a human smooth muscle (enteric type) gamma-actin cDNA.Nucleic acids research · 1990Article
- New nucleotide sequence data on the EMBL File Server.Nucleic acids research · 1989Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Mouse testis contains two size classes of actin mRNAs of 2.1 and 1.5 kilobases (kb). The 2.1-kb actin mRNA codes for cytoplasmic beta- and gamma-actin and is found throughout spermatogenesis, while the 1.5-kb actin mRNA is first detected in postmeiotic cells. Here we identify the testicular postmeiotic actin encoded by the 1.5-kb mRNA as a smooth-muscle gamma-actin (SMGA) and present its cDNA sequence. The amino acid sequence deduced from the postmeiotic actin cDNA sequence was nearly identical to that of a chicken gizzard SMGA, with one amino acid replacement at amino acid 359, where glutamine was substituted for proline. The nucleotide sequence of the untranslated region of the SMGA differed substantially from those of other isotypes of mammalian actins. By using the 3' untranslated region of the testicular SMGA, a highly specific probe was obtained. The 1.5-kb mRNA was detected in RNA from mouse aorta, small intestine, and uterus, but not in RNA isolated from mouse brain, heart, and spleen. Testicular SMGA mRNA was first detected and increased substantially in amount during spermiogenesis in the germ cells, in contrast to the decrease of the cytoplasmic beta- and gamma-actin mRNAs towards the end of spermatogenesis. Testicular SMGA mRNA was present in the polysome fractions, indicating that it was translated. These studies demonstrate the existence of an SMGA in male haploid germ cells. The implications of the existence of an SMGA in male germ cells are discussed.
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