ArticleMolecular and cellular biology1989
Interaction of nuclear proteins with a positive cis-acting element of rat embryonic myosin heavy-chain promoter: identification of a new transcriptional factor.
Article in Molecular and cellular biology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
10 citing papers in PubMed, 31 citations in OpenAlex.
- Thyroid hormone is a critical determinant for the regulation of the cochlear motor protein prestin.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- The 5'-end of the porcine perinatal myosin heavy chain gene shows alternative splicing and is clustered with repeat elements.Journal of muscle research and cell motility · 2000Article
- Transcription of the human beta enolase gene (ENO-3) is regulated by an intronic muscle-specific enhancer that binds myocyte-specific enhancer factor 2 proteins and ubiquitous G-rich-box binding factors.Molecular and cellular biology · 1995Article
- The MEF-3 motif is required for MEF-2-mediated skeletal muscle-specific induction of the rat aldolase A gene.Molecular and cellular biology · 1993Article
- MEF2C, a MADS/MEF2-family transcription factor expressed in a laminar distribution in cerebral cortex.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
- Tissue-specific restriction of skeletal muscle troponin C gene expression.Gene expression · 1993Article
- Autonomous activity of the alternate aldolase A muscle promoter is maintained by a sequestering mechanism.Nucleic acids research · 1992Article
- Compilation of vertebrate-encoded transcription factors.Nucleic acids research · 1992Article
- Mechanism of tissue-specific transcription: interplay between positive and negative regulatory factors.Gene expression · 1992Article
- A new myocyte-specific enhancer-binding factor that recognizes a conserved element associated with multiple muscle-specific genes.Molecular and cellular biology · 1989Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A DNA fragment of the rat embryonic myosin heavy-chain promoter (MHCemb) has been found to specifically bind a nuclear factor (NFe) present in extracts prepared from mouse C2 myoblasts, myotubes, and HeLa cells. The nucleotide sequence of the binding site (BSe) has been identified as 5'-GTGTCAGTCA-3' and was located between -93 and -84. Transient expression studies on MHCemb promoter deletion constructs in C2 myoblasts and C2 myotubes suggested that NFe is a transcriptional factor. Deletion of the NFe-binding site resulted in four- to sixfold and twofold reduction of promoter activity in C2 myotubes and C2 myoblasts, respectively. Furthermore, point mutations at the BSe not only abolished the NFe-binding activity of the MHCemb promoter but also resulted in reduction of the promoter activity to levels similar to those of the deletion constructs in C2 myotubes, myoblasts, and Hela cells (four- to sixfold). Although BSe and the binding site of the recently identified transcriptional factors AP-1 and ATF share significant homology, the results from competition binding assays indicated that NFe is different from both AP-1 and ATF.
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