Evidence map›Paper›PMID 27475633›Full record

ArticleJournal of assisted reproduction and genetics2016

Increasing of blastocyst rate and gene expression in co-culture of bovine embryos with adult adipose tissue-derived mesenchymal stem cells.

Moysés S Miranda, Hamilton S Nascimento, Mayra P R Costa, Nathália N Costa, Karynne N L Brito, Cinthia T A Lopes, Simone S D Santos, Marcela S Cordeiro, Otávio M Ohashi

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of assisted reproduction and genetics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed, 1 pooled it
1.2field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 1 synthesis or guideline pooled it, 27 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Review
  4. Article
  5. Review
  6. Article
  7. Article
  8. Review
  9. An Update on Applications of Cattle Mesenchymal Stromal Cells.Animals : an open access journal from MDPI · 2022
    Review
  10. Article
  11. Review
  12. Article
  13. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Moysés S MirandaLaboratório de Fecundação In Vitro, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brazil. moyses@ufpa.br.
Hamilton S NascimentoLaboratório de Fecundação In Vitro, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brazil.
Mayra P R CostaLaboratório de Células Tronco em Medicina Veterinária (LabTron-Vet), Instituto de Medicina Veterinária, Universidade Federal do Pará, Castanhal, PA, Brazil.
Nathália N CostaLaboratório de Fecundação In Vitro, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brazil.
Karynne N L BritoLaboratório de Fecundação In Vitro, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brazil.
Cinthia T A LopesLaboratório de Células Tronco em Medicina Veterinária (LabTron-Vet), Instituto de Medicina Veterinária, Universidade Federal do Pará, Castanhal, PA, Brazil.
Simone S D SantosLaboratório de Fecundação In Vitro, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brazil.
Marcela S CordeiroLaboratório de Fecundação In Vitro, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brazil.
Otávio M OhashiLaboratório de Fecundação In Vitro, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brazil.
Universidade Federal do Pará · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeDespite advances in the composition of defined embryo culture media, co-culture with somatic cells is still used for bovine in vitro embryo production (IVEP) in many laboratories worldwide. Granulosa cells are most often used for this purpose, although recent work suggests that co-culture with stem cells of adult or embryonic origin or their derived biomaterials may improve mouse, cattle, and pig embryo development. MATERIALS AND

methodsIn experiment 1, in vitro produced bovine embryos were co-cultured in the presence of two concentrations of bovine adipose tissue-derived mesenchymal cells (b-ATMSCs; 10

resultsIn experiment 1, co-culture with 10

conclusionsCo-culture of bovine embryos with b-ATMSCs was more beneficial than the traditional co-culture system with granulosa cells. We speculate that the microenvironmental modulatory potential of MSCs, by means of soluble substances and exosome secretions, could be responsible for the positive effects observed. Further experiments must be done to evaluate if this beneficial effect in vitro also translates to an increase in offspring following embryo transfer. Moreover, this study provides an interesting platform to study the basic requirements during preimplantation embryo development, which, in turn, may aid the improvement of embryo culture protocols in bovine and other species.

Indexed as

Coculture TechniquesCulture MediaMesenchymal Stem Cell TransplantationAdipose TissueAdultAnimalsBlastocystCattleEmbryonic DevelopmentFemaleGene Expression Regulation, DevelopmentalGranulosa CellsHumansMesenchymal Stem CellsMiceMonosaccharide Transport ProteinsCulture MediaMonosaccharide Transport ProteinsOctamer Transcription Factor-3BovineCo-cultureEmbryoIVEPMesenchymal stem cells

Identifiers

PMID27475633
PMCPMC5065556
OpenAlexW2480630743

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.