ArticleThe Journal of chemical physics2016
Communication: Contrasting effects of glycerol and DMSO on lipid membrane surface hydration dynamics and forces.
Article in The Journal of chemical physics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it.
- A scalable outpatient framework for BI-RADS 2-4B breast nodule management: an iodine-DMSO transdermal protocol.Frontiers in health services · 2026Pooled it
- Whole-Cell Glycerol-free Fluorescence Cryo-Spectroscopy Reveals Electronic Excitation Dynamics in the DiatomJACS Au · 2026Article
- Bimodal Cholesterol for Correlative In-Cell DNP Solid-State NMR and Confocal Microscopy of the Plasma Membrane.Journal of the American Chemical Society · 2026Article
- Spontaneous nanosized liposome formation from crude dried lecithin upon addition of glycerol.Scientific reports · 2024Article
- Structure and function of skin barrier lipids: Effects of hydration and natural moisturizers in vitro.Biophysical journal · 2024Article
- Efficacy assessment of different cryoprotectants for preserving the viability of Enterobacterales strains at - 20 °C.Scientific reports · 2024Article
- Dehydration does not affect lipid-based hydration lubrication.Nanoscale · 2022Article
- The Role of Cryoprotective Agents in Liposome Stabilization and Preservation.International journal of molecular sciences · 2022Review
- Exposure to Nepalese Propolis Alters the Metabolic State ofFrontiers in microbiology · 2022Article
- Carrier Solvents of Electronic Nicotine Delivery Systems Alter Pulmonary Surfactant.Chemical research in toxicology · 2021Article
- In-Cell NMR of Intact Mammalian Cells Preserved with the Cryoprotectants DMSO and Glycerol Have Similar DNP Performance.Frontiers in molecular biosciences · 2021Article
- Biomolecular Perturbations in In-Cell Dynamic Nuclear Polarization Experiments.Frontiers in molecular biosciences · 2021Article
- Dynamic Nuclear Polarization of Biomembrane Assemblies.Biomolecules · 2020Article
- Effect of DMSO on the Mechanical and Structural Properties of Model and Biological Membranes.Biophysical journal · 2020Article
- The roles and applications of chaotropes and kosmotropes in industrial fermentation processes.World journal of microbiology & biotechnology · 2020Review
- Untargetted Metabolomic Exploration of theBiomolecules · 2020Article
- AFM nanoindentation reveals decrease of elastic modulus of lipid bilayers near freezing point of water.Scientific reports · 2019Article
- A biradical-tagged phospholipid as a polarizing agent for solid-state MAS Dynamic Nuclear Polarization NMR of membrane proteins.Solid state nuclear magnetic resonance · 2019Article
- Surface chemical heterogeneity modulates silica surface hydration.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- A single-molecule assessment of the protective effect of DMSO against DNA double-strand breaks induced by photo-and γ-ray-irradiation, and freezing.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Glycerol and dimethyl sulfoxide (DMSO) are commonly used cryoprotectants in cellular systems, but due to the challenges of measuring the properties of surface-bound solvent, fundamental questions remain regarding the concentration, interactions, and conformation of these solutes at lipid membrane surfaces. We measured the surface water diffusivity at gel-phase dipalmitoylphosphatidylcholine (DPPC) bilayer surfaces in aqueous solutions containing ≤7.5 mol. % of DMSO or glycerol using Overhauser dynamic nuclear polarization. We found that glycerol similarly affects the diffusivity of water near the bilayer surface and that in the bulk solution (within 20%), while DMSO substantially increases the diffusivity of surface water relative to bulk water. We compare these measurements of water dynamics with those of equilibrium forces between DPPC bilayers in the same solvent mixtures. DMSO greatly decreases the range and magnitude of the repulsive forces between the bilayers, whereas glycerol increases it. We propose that the differences in hydrogen bonding capability of the two solutes leads DMSO to dehydrate the lipid head groups, while glycerol affects surface hydration only as much as it affects the bulk water properties. The results suggest that the mechanism of the two most common cryoprotectants must be fundamentally different: in the case of DMSO by decoupling the solvent from the lipid surface, and in the case of glycerol by altering the hydrogen bond structure and intermolecular cohesion of the global solvent, as manifested by increased solvent viscosity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.