ArticleBiosensors & bioelectronics2016
In situ, dual-mode monitoring of organ-on-a-chip with smartphone-based fluorescence microscope.
Article in Biosensors & bioelectronics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 1 synthesis or guideline pooled it, 78 citations in OpenAlex.
- 3D Printing Techniques and Their Applications to Organ-on-a-Chip Platforms: A Systematic Review.Sensors (Basel, Switzerland) · 2021Pooled it
- Integrated microoptical system for continuous fluorescence monitoring of microtissues.Microsystems & nanoengineering · 2025Article
- Enhancing Biomarker Detection and Imaging Performance of Smartphone Fluorescence Microscopy Devices.Biosensors · 2025Article
- Recent Advances of PDMS In Vitro Biomodels for Flow Visualizations and Measurements: From Macro to Nanoscale Applications.Micromachines · 2024Review
- Microphysiological system with integrated sensors to study the effect of pulsed electric field.Scientific reports · 2024Article
- Advances and challenges in organ-on-chip technology: toward mimicking human physiology and disease in vitro.Medical & biological engineering & computing · 2024Review
- A holistic analysis of the intrinsic and delivery-mediated toxicity of siRNA therapeutics.Advanced drug delivery reviews · 2023Review
- Review
- Optical Biosensors for the Diagnosis of COVID-19 and Other Viruses-A Review.Diagnostics (Basel, Switzerland) · 2023Review
- Integrated biosensors for monitoring microphysiological systems.Lab on a chip · 2022Review
- Toward a modular, integrated, miniaturized, and portable microfluidic flow control architecture for organs-on-chips applications.Biomicrofluidics · 2022Article
- Microscopic Imaging Methods for Organ-on-a-Chip Platforms.Micromachines · 2022Review
- Organ-on-a-Chip Platforms for Drug Screening and Delivery in Tumor Cells: A Systematic Review.Cancers · 2022Review
- Quantitative Imaging of Bacteriophage Amplification for Rapid Detection of Bacteria in Model Foods.Frontiers in microbiology · 2022Article
- Review
- Organ-on-a-chip platforms for accelerating the evaluation of nanomedicine.Bioactive materials · 2021Review
- Organ-on-a-Chip systems for new drugs development.ADMET & DMPK · 2021Review
- Innovations in 3-Dimensional Tissue Models of Human Brain Physiology and Diseases.Advanced functional materials · 2020Article
- Instrumented Microphysiological Systems for Real-Time Measurement and Manipulation of Cellular Electrochemical Processes.iScience · 2019Review
- Methods of Delivering Mechanical Stimuli to Organ-on-a-Chip.Micromachines · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The use of organ-on-a-chip (OOC) platforms enables improved simulation of the human kidney's response to nephrotoxic drugs. The standard method of analyzing nephrotoxicity from existing OOC has majorly consisted of invasively collecting samples (cells, lysates, media, etc.) from an OOC. Such disruptive analyses potentiate contamination, disrupt the replicated in vivo environment, and require expertize to execute. Moreover, traditional analyses, including immunofluorescence microscopy, immunoblot, and microplate immunoassay are essentially not in situ and require substantial time, resources, and costs. In the present work, the incorporation of fluorescence nanoparticle immunocapture/immunoagglutination assay into an OOC enabled dual-mode monitoring of drug-induced nephrotoxicity in situ. A smartphone-based fluorescence microscope was fabricated as a handheld in situ monitoring device attached to an OOC. Both the presence of γ-glutamyl transpeptidase (GGT) on the apical brush-border membrane of 786-O proximal tubule cells within the OOC surface, and the release of GGT to the outflow of the OOC were evaluated with the fluorescence scatter detection of captured and immunoagglutinated anti-GGT conjugated nanoparticles. This dual-mode assay method provides a novel groundbreaking tool to enable the internal and external in situ monitoring of the OOC, which may be integrated into any existing OOCs to facilitate their subsequent analyses.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.