Evidence map›Paper›PMID 27474881›Full record

ArticleHepatology (Baltimore, Md.)2017

Extracellular vesicles carry microRNA-195 to intrahepatic cholangiocarcinoma and improve survival in a rat model.

Ling Li, Klaus Piontek, Masaharu Ishida, Michel Fausther, Jonathan A Dranoff, Rongdang Fu, Esteban Mezey, Stephen J Gould, Francis K Fordjour, Stephen J Meltzer and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Hepatology (Baltimore, Md.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 83 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
83citing papers in PubMed, 1 pooled it
7.7field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

83 citing papers in PubMed, 1 synthesis or guideline pooled it, 142 citations in OpenAlex.

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23 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

12 authors at 6 institutions in 2 countries.

Ling LiDivision of Gastroenterology and Hepatology, School of Medicine, The Johns Hopkins University, Baltimore, MD.
Klaus PiontekDivision of Gastroenterology and Hepatology, School of Medicine, The Johns Hopkins University, Baltimore, MD.
Masaharu IshidaDivision of Gastroenterology and Hepatology, School of Medicine, The Johns Hopkins University, Baltimore, MD.
Michel FaustherDivision of Gastroenterology and Hepatology, University of Arkansas for Medical Sciences, Little Rock, AR.
Jonathan A DranoffDivision of Gastroenterology and Hepatology, University of Arkansas for Medical Sciences, Little Rock, AR.
Rongdang FuDivision of Gastroenterology and Hepatology, School of Medicine, The Johns Hopkins University, Baltimore, MD.
Esteban MezeyDivision of Gastroenterology and Hepatology, School of Medicine, The Johns Hopkins University, Baltimore, MD.
Stephen J GouldDepartment of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, MD.
Francis K FordjourDepartment of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, MD.
Stephen J MeltzerDivision of Gastroenterology and Hepatology, School of Medicine, The Johns Hopkins University, Baltimore, MD.
Alphonse E SiricaDivision of Cellular and Molecular Pathogenesis, Department of Pathology, Virginia Commonwealth University School of Medicine, Richmond, VA.
Florin M SelaruDivision of Gastroenterology and Hepatology, School of Medicine, The Johns Hopkins University, Baltimore, MD.
Johns Hopkins Medicine · USJohns Hopkins University · USUniversity of Arkansas for Medical Sciences · USSidney Kimmel Cancer Center · USTohoku University · JPVirginia Commonwealth University · US

Funding

Translational Research Enhancement CoreP30DK089502 · NIDDK · JOHNS HOPKINS UNIVERSITY · PI DONOWITZ, MARK · 2011 to 2020
$12.0M
ALTERED GROWTH FACTOR PATHWAYS IN BILLIARY CANCERR01CA083650 · NCI · VIRGINIA COMMONWEALTH UNIVERSITY · PI SIRICA, ALPHONSE E · 2000 to 2017
$4.4M
Regulation of Hepatic Stellate Cells by Extracellular NucleotidesR01DK076735 · NIDDK · YALE UNIVERSITY · PI DRANOFF, JONATHAN A · 2008 to 2012
$1.8M
MicroRNA involvement in the regulation of biliary epitheliumK08DK090154 · NIDDK · JOHNS HOPKINS UNIVERSITY · PI SELARU, FLORIN · 2011 to 2015
$706k
Pathogenesis of Biliary CirrhosisR56DK070849 · NIDDK · YALE UNIVERSITY · PI DRANOFF, JONATHAN A · 2010 to 2010
$386k
NCI NIH HHS R01 CA083650NIDDK NIH HHS K08 DK090154NIDDK NIH HHS P30 DK089502NIDDK NIH HHS R01 DK076735NIDDK NIH HHS R56 DK070849
6 · The paper itself

Abstract

The cancer microenvironment plays a central role in cancer development, growth, and homeostasis. This paradigm suggests that cancer fibroblasts support cancers, probably in response to stimuli received from the cancer cells. We aimed at investigating whether extracellular vesicles (EVs) can shuttle microRNA (miR) species between cancer-associated fibroblasts (CAFs) and cancer cells. To this end, we extracted EVs according to published protocols. EVs were studied for their miR content by quantitative reverse-transcription polymerase chain reaction. EVs were transfected with select miR species and utilized in vitro as well as in vivo in a rat model of cholangiocarcinoma (CCA). We found that miR-195 is down-regulated in CCA cells, as well as in adjoining fibroblasts. Furthermore, we report that EVs shuttle miR-195 from fibroblasts to cancer cells. Last, we show that fibroblast-derived EVs, loaded with miR-195, can be administered in a rat model of CCA, concentrate within the tumor, decrease the size of cancers, and improve survival of treated rats.

conclusionEVs play a salient role in trafficking miR species between cancer cells and CAFs in human CCA. Understanding of these mechanisms may allow devising of novel therapeutics. (Hepatology 2017;65:501-514).

Indexed as

AnimalsBile Duct NeoplasmsCarcinogenesisCell MovementCholangiocarcinomaDisease Models, AnimalDown-RegulationExtracellular VesiclesFibroblastsHumansImmunohistochemistryMaleMicroRNAsRandom AllocationRatsRats, Inbred F344MicroRNAs

Identifiers

PMID27474881
PMCPMC5258762
OpenAlexW2477709189

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.