ArticleGenetics2016
Divergent Transactivation of Maize Storage Protein Zein Genes by the Transcription Factors Opaque2 and OHPs.
Article in Genetics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 44 citations in OpenAlex.
- ZmMYB127 Modulates Maize Kernel Texture and Size by Integrating the Synthesis of Starch, Zein Proteins and Auxin.Plant biotechnology journal · 2026Article
- From development to yield: genetic and molecular regulation of agronomic traits in maize seeds.Frontiers in plant science · 2026Review
- Decades' progress and prospects on maize functional genomics and molecular breeding.Science China. Life sciences · 2025Review
- Review
- Identification of QTLs associated withFrontiers in plant science · 2025Article
- Temporal profile of amino acids and protein fractions in the developing kernel of maize germplasm.Scientific reports · 2024Article
- HEAT SHOCK PROTEIN 90.6 interacts with carbon and nitrogen metabolism components during seed development.Plant physiology · 2023Article
- ABA-induced phosphorylation of basic leucine zipper 29, ABSCISIC ACID INSENSITIVE 19, and Opaque2 by SnRK2.2 enhances gene transactivation for endosperm filling in maize.The Plant cell · 2022Article
- Maize RNA 3'-terminal phosphate cyclase-like protein promotes 18S pre-rRNA cleavage and is important for kernel development.The Plant cell · 2022Article
- Regulators of Starch Biosynthesis in Cereal Crops.Molecules (Basel, Switzerland) · 2021Review
- Trihelix Transcription FactorInternational journal of molecular sciences · 2021Article
- The B3 domain-containing transcription factor ZmABI19 coordinates expression of key factors required for maize seed development and grain filling.The Plant cell · 2021Article
- Maize kernel development.Molecular breeding : new strategies in plant improvement · 2021Review
- European maize genomes highlight intraspecies variation in repeat and gene content.Nature genetics · 2020Article
- The regulation of zein biosynthesis in maize endosperm.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Review
- NAC-type transcription factors regulate accumulation of starch and protein in maize seeds.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Stability Analysis of Kernel Quality Traits in Exotic-Derived Doubled Haploid Maize Lines.The plant genome · 2019Article
- An Exome-seq Based Tool for Mapping and Selection of Candidate Genes in Maize Deletion Mutants.Genomics, proteomics & bioinformatics · 2018Article
- Opaque-2 Regulates a Complex Gene Network Associated with Cell Differentiation and Storage Functions of Maize Endosperm.The Plant cell · 2018Article
- The ZmbZIP22 Transcription Factor Regulates 27-kD γ-Zein Gene Transcription during Maize Endosperm Development.The Plant cell · 2018Article
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3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Maize transcription factors (TFs) opaque2 (O2) and the O2 heterodimerizing proteins (OHP1 and OHP2) originated from an ancient segmental duplication. The 22-kDa (z1C) and 19-kDa (z1A, z1B, and z1D) α-zeins are the most abundant storage proteins in maize endosperm. O2 is known to regulate α-zein gene expression, but its target motifs in the 19-kDa α-zein gene promoters have not been identified. The mechanisms underlying the regulation of α-zein genes by these TFs are also not well understood. In this study, we found that the O2 binding motifs in the α-zein gene promoters are quite flexible, with ACGT being present in the z1C and z1A promoters and a variant, ACAT, being present in the z1B and z1D promoters. OHPs recognized and transactivated all of the α-zein promoters, although to much lower levels than did O2. In the presence of O2, the suppression of OHPs did not cause a significant reduction in the transcription of α-zein genes, but in the absence of O2, OHPs were critical for the expression of residual levels of α-zeins. These findings demonstrated that O2 is the primary TF and that OHPs function as minor TFs in this process. This relationship is the converse of that involved in 27-kDa γ-zein gene regulation, indicating that the specificities of O2 and the OHPs for regulating zein genes diverged after gene duplication. The prolamine-box binding factor by itself has limited transactivation activity, but it promotes the binding of O2 to O2 motifs, resulting in the synergistic transactivation of α-zein genes.
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