ArticleGenes & development2016
Loss of l(3)mbt leads to acquisition of the ping-pong cycle in Drosophila ovarian somatic cells.
Article in Genes & development, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 39 citations in OpenAlex.
- Article
- Host-transposable element coexistence: a matter of resistance, tolerance and trade-off.The EMBO journal · 2026Review
- piRNA processing within non-membrane structures is governed by constituent proteins and their functional motifs.The FEBS journal · 2025Review
- Traffic Jam activates the Flamenco piRNA cluster locus and the Piwi pathway to ensure transposon silencing and Drosophila fertility.Cell reports · 2025Article
- Transcriptional regulation of the piRNA pathway by Ovo in animal ovarian germ cells.Genes & development · 2025Article
- Themes and variations on piRNA-guided transposon control.Mobile DNA · 2023Review
- The NSL complex is required for piRNA production from telomeric clusters.Life science alliance · 2023Article
- Lint-O cooperates with L(3)mbt in target gene suppression to maintain homeostasis in fly ovary and brain.EMBO reports · 2022Article
- Article
- piRNA- and siRNA-mediated transcriptional repression in Drosophila, mice, and yeast: new insights and biodiversity.EMBO reports · 2021Review
- A mosquito small RNA genomics resource reveals dynamic evolution and host responses to viruses and transposons.Genome research · 2021Article
- Modulation of Aub-TDRD interactions elucidates piRNA amplification and germplasm formation.Life science alliance · 2021Article
- Essential roles of Windei and nuclear monoubiquitination of Eggless/SETDB1 in transposon silencing.EMBO reports · 2019Article
- Assembly and Function of Gonad-Specific Non-Membranous Organelles inNon-coding RNA · 2019Review
- Nuclear RNA export factor variant initiates piRNA-guided co-transcriptional silencing.The EMBO journal · 2019Article
- The histone code reader PHD finger protein 7 controls sex-linked disparities in gene expression and malignancy inScience advances · 2019Article
- Requirements for multivalent Yb body assembly in transposon silencing in Drosophila.EMBO reports · 2019Article
- Generating and working with Drosophila cell cultures: Current challenges and opportunities.Wiley interdisciplinary reviews. Developmental biology · 2019Review
- Thawing, Culturing, and Cryopreserving Drosophila Cell Lines.Journal of visualized experiments : JoVE · 2019Article
- piRNA-Guided Genome Defense: From Biogenesis to Silencing.Annual review of genetics · 2018Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Drosophila germ cells, PIWI-interacting RNAs (piRNAs) are amplified through a PIWI slicer-dependent feed-forward loop termed the ping-pong cycle, yielding secondary piRNAs. However, the detailed mechanism remains poorly understood, largely because an ex vivo model system amenable to biochemical analyses has not been available. Here, we show that CRISPR-mediated loss of function of lethal (3) malignant brain tumor [l(3)mbt] leads to ectopic activation of the germ-specific ping-pong cycle in ovarian somatic cells. Perinuclear foci resembling nuage, the ping-pong center, appeared following l(3)mbt mutation. This activation of the ping-pong machinery in cultured cells will greatly facilitate elucidation of the mechanism underlying secondary piRNA biogenesis in Drosophila.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.