ArticleTalanta2016
Fast, ultra-trace detection of juvenile hormone III from mosquitoes using mass spectrometry.
Article in Talanta, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed.
- Juvenile hormone signaling acts during development and post-mating to suppress antibacterial defenses in Drosophila melanogaster.Scientific reports · 2026Article
- One genome, multiple phenotypes: decoding the evolution and mechanisms of environmentally induced developmental plasticity in insects.Biochemical Society transactions · 2023Review
- Evaluation of Antioxidant Activity and Biotransformation ofMolecules (Basel, Switzerland) · 2022Article
- Epoxidation of juvenile hormone was a key innovation improving insect reproductive fitness.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Following de novo triglyceride dynamics in ovaries of Aedes aegypti during the previtellogenic stage.Scientific reports · 2021Article
- Approaches and Tools to Study the Roles of Juvenile Hormones in Controlling Insect Biology.Insects · 2020Review
- A mosquito juvenile hormone binding protein (mJHBP) regulates the activation of innate immune defenses and hemocyte development.PLoS pathogens · 2020Article
- Common structural features facilitate the simultaneous identification and quantification of the five most common juvenile hormones by liquid chromatography-tandem mass spectrometry.Insect biochemistry and molecular biology · 2020Article
- Inhibition of juvenile hormone synthesis in mosquitoes by the methylation inhibitor 3-deazaneplanocin A (DZNep).Insect biochemistry and molecular biology · 2019Article
- Juvenile hormone controls ovarian development in female Anopheles albimanus mosquitoes.Scientific reports · 2019Article
- JH biosynthesis and hemolymph titers in adult male Aedes aegypti mosquitoes.Insect biochemistry and molecular biology · 2018Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
In the present work, a new protocol for fast separation and quantification of JH III from biological samples using liquid chromatography coupled to electrospray tandem mass spectrometry is described. In particular, the proposed protocol improves existing methodologies by combining a limited number of sample preparation steps with fast LC-MS/MS detection, providing lower limits of detection and demonstrated matrix effect control, together with high inter and intraday reproducibility. A limit of detection of 8pg/mL (0.32pg on column) was achieved, representing a 15-fold gain in sensitivity with respect to previous LC-MS based protocols. The performance of the LC-MS/MS protocol is comparable to previously described JH III quantitation protocol based on fluorescence detection, with the added advantage that quantification is independent of the availability of fluorescent tags that are often unavailable or show quite diverse responses on a batch-to-batch basis. Additionally, a detailed description of the JH III fragmentation pathway is provided for the first time, based on isolation of the molecular ion and their intermediate fragments using in-source MS/MS, MS/MS(n) and FT-ICR MS/MS measurements. The JH III workflow was evaluated as a function of developmental changes, sugar feeding and farnesoic acid stimulation in mosquitoes and can be applied to the detection of other juvenile hormones.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.