ArticlePlant physiology2016
Class I and II Small Heat Shock Proteins Together with HSP101 Protect Protein Translation Factors during Heat Stress.
Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers.
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Who cites it
82 citing papers in PubMed, 143 citations in OpenAlex.
- Principles and mechanisms of plant acclimation to heat stress.Nature reviews. Molecular cell biology · 2026Review
- Gene Families, Structural and Functional Contexts Shape Protein Thermostability and Paralog Divergence inPlants (Basel, Switzerland) · 2026Article
- Article
- Small heat shock and J-domain proteins direct defense against areca palm velarivirus 1 by degrading coat protein via autophagy.Nature communications · 2026Article
- 2-Cys peroxiredoxins and the chaperone cpHSP70 act in concert in chloroplast biogenesis in Arabidopsis seedlings.The Plant cell · 2026Article
- Molecular Insights into the Role ofPlants (Basel, Switzerland) · 2026Article
- Transcription fidelity and control of alternative splicing contribute to heat stress survival in Arabidopsis.The Plant cell · 2025Article
- Responses of grapevine cells to physiological doses of ethanol, including induced resistance to heat stress.Plant biology (Stuttgart, Germany) · 2025Article
- Heterologous WatermelonCurrent issues in molecular biology · 2025Article
- Translational reprogramming under heat stress: a plant's perspective.Royal Society open science · 2025Review
- Role of heat shock proteins and analysis of gene expression in response to high temperatures in date palm (Phoenix dactylifera).Genes & genomics · 2025Article
- A thermosensor FUST1 primes heat-induced stress granule formation via biomolecular condensation in Arabidopsis.Cell research · 2025Article
- Cooperative condensation of RNA-DIRECTED DNA METHYLATION 16 splicing isoforms enhances heat tolerance in Arabidopsis.Nature communications · 2025Article
- Insights into the Heat Shock Protein 70 (Hsp70) Family inPlants (Basel, Switzerland) · 2024Article
- Infrared laser-induced gene expression in single cells characterized by quantitative imaging in Physcomitrium patens.Communications biology · 2024Article
- Identification of the Novel Small Compound Stress Response Regulators 1 and 2 That Affect Plant Abiotic Stress Signaling.Biomolecules · 2024Article
- Establishment and Maintenance of Heat-Stress Memory in Plants.International journal of molecular sciences · 2024Review
- Comparative proteomics in tall fescue to reveal underlying mechanisms for improving Photosystem II thermotolerance during heat stress memory.BMC genomics · 2024Article
- Heat shock proteinPhysiology and molecular biology of plants : an international journal of functional plant biology · 2024Article
- Review
22 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ubiquitous small heat shock proteins (sHSPs) are well documented to act in vitro as molecular chaperones to prevent the irreversible aggregation of heat-sensitive proteins. However, the in vivo activities of sHSPs remain unclear. To investigate the two most abundant classes of plant cytosolic sHSPs (class I [CI] and class II [CII]), RNA interference (RNAi) and overexpression lines were created in Arabidopsis (Arabidopsis thaliana) and shown to have reduced and enhanced tolerance, respectively, to extreme heat stress. Affinity purification of CI and CII sHSPs from heat-stressed seedlings recovered eukaryotic translation elongation factor (eEF) 1B (α-, β-, and γ-subunits) and eukaryotic translation initiation factor 4A (three isoforms), although the association with CI sHSPs was stronger and additional proteins involved in translation were recovered with CI sHSPs. eEF1B subunits became partially insoluble during heat stress and, in the CI and CII RNAi lines, showed reduced recovery to the soluble cell fraction after heat stress, which was also dependent on HSP101. Furthermore, after heat stress, CI sHSPs showed increased retention in the insoluble fraction in the CII RNAi line and vice versa. Immunolocalization revealed that both CI and CII sHSPs were present in cytosolic foci, some of which colocalized with HSP101 and with eEF1Bγ and eEF1Bβ. Thus, CI and CII sHSPs have both unique and overlapping functions and act either directly or indirectly to protect specific translation factors in cytosolic stress granules.
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Registered trials
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