Evidence map›Paper›PMID 27473904›Full record

ArticleMethods in cell biology2016

Visualization of in vivo septin ultrastructures by platinum replica electron microscopy.

K Ong, T Svitkina, E Bi

Open access · greenAbstract read
In one paragraph

Article in Methods in cell biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.5field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 12 citations in OpenAlex.

  1. Curvature sensing as an emergent property of multiscale assembly of septins.Proceedings of the National Academy of Sciences of the United States of America · 2023
    Article
  2. Imaging Cytoskeleton Components by Electron Microscopy.Methods in molecular biology (Clifton, N.J.) · 2022
    Article
  3. The TPLATE complex mediates membrane bending during plant clathrin-mediated endocytosis.Proceedings of the National Academy of Sciences of the United States of America · 2021
    Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

K OngUniversity of Pennsylvania, Philadelphia, PA, United States.
T SvitkinaUniversity of Pennsylvania, Philadelphia, PA, United States.
E BiUniversity of Pennsylvania, Philadelphia, PA, United States.
University of Pennsylvania · US

Funding

TRAINING PROGRAM IN CELL AND MOLECULAR BIOLOGYT32GM007229 · NIGMS · UNIVERSITY OF PENNSYLVANIA · PI BARTOLOMEI, MARISA S. · 1985 to 2023
$14.0M
Graduate Training in Developmental BiologyT32HD083185 · NICHD · UNIVERSITY OF PENNSYLVANIA · PI Michael Granato, Foteini Mourkioti · 2015 to 2026
$4.0M
Mechanistic Analysis of Cytokinesis in EukaryotesR01GM115420 · NIGMS · UNIVERSITY OF PENNSYLVANIA · PI BI, ERFEI · 2015 to 2022
$3.7M
Multifaceted roles of nonmuscle myosin II in cell adhesion and migrationR01GM095977 · NIGMS · UNIVERSITY OF PENNSYLVANIA · PI SVITKINA, TATYANA · 2013 to 2020
$2.7M
NICHD NIH HHS T32 HD083185NIGMS NIH HHS R01 GM095977NIGMS NIH HHS R01 GM115420NIGMS NIH HHS T32 GM007229
6 · The paper itself

Abstract

Septins are cytoskeletal proteins involved in diverse biological processes including cytokinesis, cell morphogenesis, motility, and ciliogenesis. Septins form various filamentous structures in vitro and in vivo, but the higher-order architecture of septin structures in vivo remains poorly defined. The best understood system in this respect is the budding yeast Saccharomyces cerevisiae, where septins form a ring structure that undergoes multiple stages of remodeling during the cell cycle. In this chapter, we describe a method for visualizing supramolecular septin structures in yeast at high spatial resolution using platinum replica electron microscopy. This approach can be applied to further understand the regulation of assembly and remodeling of septin higher-order structures, as well as the relationship between septin architecture and function.

Indexed as

Cell Cycle ProteinsCytokinesisMicroscopy, ElectronMultiprotein ComplexesPlatinumSaccharomyces cerevisiaeSeptinsCell Cycle ProteinsMultiprotein ComplexesPlatinumSeptinsCytokinesisCytoskeletonElectron microscopyS. cerevisiaeSeptin

Identifiers

PMID27473904
PMCPMC5497467
OpenAlexW2482443502

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.