ArticleJournal of experimental botany2016
The role of Arabidopsis Actin-Related Protein 3 in amyloplast sedimentation and polar auxin transport in root gravitropism.
Article in Journal of experimental botany, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 45 citations in OpenAlex.
- Both brefeldin A and latrunculin B treatment induces cytoplasmic aggregation of plasma membrane-associated proteins in epidermal cells, but not in columella cells.Plant signaling & behavior · 2026Article
- Actin depolymerization by Latrunculin B can either suppress or promote root gravitropism, depending on the developmental stages in Arabidopsis.Plant signaling & behavior · 2025Article
- Article
- Intracellular MIZU-KUSSEI1 movement and hydrotropism in Arabidopsis require F-actin organization.Plant physiology · 2025Article
- Filamentous Temperature-Sensitive Z Protein J175 Regulates Maize Chloroplasts' and Amyloplasts' Division and Development.Plants (Basel, Switzerland) · 2025Article
- Exploring plant responses to altered gravity for advancing space agriculture.Plant communications · 2025Review
- Synergism of vesicle trafficking and cytoskeleton during regulation of plant growth and development: A mechanistic outlook.Heliyon · 2023Review
- Exploring the Role of the Plant Actin Cytoskeleton: From Signaling to Cellular Functions.International journal of molecular sciences · 2023Review
- Gravity sensing and responses in the coordination of the shoot gravitropic setpoint angle.The New phytologist · 2022Review
- Identification of loci controlling timing of stem elongation in red clover using genotyping by sequencing of pooled phenotypic extremes.Molecular genetics and genomics : MGG · 2022Article
- Blocking IbmiR319a Impacts Plant Architecture and Reduces Drought Tolerance in Sweet Potato.Genes · 2022Article
- Spatial and temporal localization of SPIRRIG and WAVE/SCAR reveal roles for these proteins in actin-mediated root hair development.The Plant cell · 2021Article
- A minus-end directed kinesin motor directs gravitropism in Physcomitrella patens.Nature communications · 2021Article
- Latrunculin B facilitates gravitropic curvature of Arabidopsis root by inhibiting cell elongation, especially the cells in the lower flanks of the transition and elongation zones.Plant signaling & behavior · 2021Article
- Gravity sensing in plant and animal cells.NPJ microgravity · 2021Review
- Bundling up the Role of the Actin Cytoskeleton in Primary Root Growth.Frontiers in plant science · 2021Review
- Reference genes for quantitative real-time PCR normalization of Cenostigma pyramidale roots under salt stress and mycorrhizal association.Genetics and molecular biology · 2021Article
- Integrated transcriptome and miRNA analysis uncovers molecular regulators of aerial stem-to-rhizome transition in the medical herb Gynostemma pentaphyllum.BMC genomics · 2019Article
- Involvement of BIG5 and BIG3 in BRI1 Trafficking Reveals Diverse Functions of BIG-subfamily ARF-GEFs in Plant Growth and Gravitropism.International journal of molecular sciences · 2019Article
- Root Tropisms: Investigations on Earth and in Space to Unravel Plant Growth Direction.Frontiers in plant science · 2019Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gravitropism is vital for shaping directional plant growth in response to the forces of gravity. Signals perceived in the gravity-sensing cells can be converted into biochemical signals and transmitted. Sedimentation of amyloplasts in the columella cells triggers asymmetric auxin redistribution in root tips, leading to downward root growth. The actin cytoskeleton is thought to play an important role in root gravitropism, although the molecular mechanism has not been resolved. DISTORTED1 (DIS1) encodes the ARP3 subunit of the Arabidopsis Actin-Related Protein 2/3 (ARP2/3) complex, and the ARP3/DIS1 mutant dis1-1 showed delayed root curvature after gravity stimulation. Microrheological analysis revealed that the high apparent viscosity within dis1-1 central columella cells is closely associated with abnormal movement trajectories of amyloplasts. Analysis using a sensitive auxin input reporter DII-VENUS showed that asymmetric auxin redistribution was reduced in the root tips of dis1-1, and the actin-disrupting drug Latrunculin B increased the asymmetric auxin redistribution. An uptake assay using the membrane-selective dye FM4-64 indicated that endocytosis was decelerated in dis1-1 root epidermal cells. Treatment and wash-out with Brefeldin A, which inhibits protein transport from the endoplasmic reticulum to the Golgi apparatus, showed that cycling of the auxin-transporter PIN-FORMED (PIN) proteins to the plasma membrane was also suppressed in dis1-1 roots. The results reveal that ARP3/DIS1 acts in root gravitropism by affecting amyloplast sedimentation and PIN-mediated polar auxin transport through regulation of PIN protein trafficking.
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