Evidence map›Paper›PMID 27473559›Full record

ArticleOsteoarthritis and cartilage2016

Tendon and ligament as novel cell sources for engineering the knee meniscus.

P Hadidi, N K Paschos, B J Huang, A Aryaei, J C Hu, K A Athanasiou

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Osteoarthritis and cartilage, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.6field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 10 citations in OpenAlex.

  1. Advances in Regenerative Sports Medicine Research.Frontiers in bioengineering and biotechnology · 2022
    Review
  2. Article
  3. Review
  4. Article
  5. Inflammation and its resolution and the musculoskeletal system.Journal of orthopaedic translation · 2017
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

P HadidiDepartment of Biomedical Engineering, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA.
N K PaschosDepartment of Biomedical Engineering, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA.
B J HuangDepartment of Biomedical Engineering, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA.
A AryaeiDepartment of Biomedical Engineering, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA.
J C HuDepartment of Biomedical Engineering, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA.
K A AthanasiouDepartment of Biomedical Engineering, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA; Department of Orthopedic Surgery, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA. Electronic address: athanasiou@ucdavis.edu.
University of California, Davis · US

Funding

Regenerating the fibrocartilage spectrum of the TMJ: from disc to condyleR01DE019666 · NIDCR · UNIVERSITY OF CALIFORNIA AT DAVIS · PI ATHANASIOU, KYRIACOS A · 2010 to 2013
$1.4M
NIDCR NIH HHS R01 DE019666
6 · The paper itself

Abstract

objectiveThe application of cell-based therapies in regenerative medicine is hindered by the difficulty of acquiring adequate numbers of competent cells. For the knee meniscus in particular, this may be solved by harvesting tissue from neighboring tendons and ligaments. In this study, we have investigated the potential of cells from tendon and ligament, as compared to meniscus cells, to engineer scaffold-free self-assembling fibrocartilage.

methodSelf-assembling meniscus-shaped constructs engineered from a co-culture of articular chondrocytes and either meniscus, tendon, or ligament cells were cultured for 4 weeks with TGF-β1 in serum-free media. After culture, constructs were assessed for their mechanical properties, histological staining, gross appearance, and biochemical composition including cross-link content. Correlations were performed to evaluate relationships between biochemical content and mechanical properties.

resultsIn terms of mechanical properties as well as biochemical content, constructs engineered using tenocytes and ligament fibrocytes were found to be equivalent or superior to constructs engineered using meniscus cells. Furthermore, cross-link content was found to be correlated with engineered tissue tensile properties.

conclusionTenocytes and ligament fibrocytes represent viable cell sources for engineering meniscus fibrocartilage using the self-assembling process. Due to greater cross-link content, fibrocartilage engineered with tenocytes and ligament fibrocytes may maintain greater tensile properties than fibrocartilage engineered with meniscus cells.

Indexed as

LigamentsTendonsCells, CulturedChondrocytesHumansMeniscusTissue EngineeringKnee surgeryMeniscus fibrocartilagePrimary cell sourcesSelf-assembling processTissue engineering

Identifiers

PMID27473559
PMCPMC5107319
OpenAlexW2485936927

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.