ArticlePlant cell reports2016
An improved immunofluorescence staining method for chloroplast proteins.
Article in Plant cell reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Evolution and Functional Differentiation of the C-terminal Motifs of FtsZs During Plant Evolution.Molecular biology and evolution · 2024Article
- Immunofluorescence staining of chloroplast proteins with frozen sections of plant tissues.Plant cell reports · 2024Article
- Mutation of CsARC6 affects fruit color and increases fruit nutrition in cucumber.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Improvements for Tissue-Chopping-Based Immunofluorescence Staining Method of Chloroplast Proteins.Plants (Basel, Switzerland) · 2023Article
- Structural and functional insights into the chloroplast division site regulators PARC6 and PDV1 in the intermembrane space.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Keloid Core Factor CTRP3 Overexpression Significantly Controlled TGF-Disease markers · 2023Article
- A novel amphiphilic motif at the C-terminus of FtsZ1 facilitates chloroplast division.The Plant cell · 2022Article
- A Tissue-Chopping Based Immunofluorescence Staining Method for Chloroplast Proteins.Frontiers in plant science · 2022Article
- PDV2 has a dosage effect on chloroplast division in Arabidopsis.Plant cell reports · 2017Article
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Authors and funding
5 authors.
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Abstract
key messageAn improved immunofluorescence staining method significantly facilitates the visualization of the subcellular localization of interested proteins in chloroplasts. As an important technical approach, immunofluorescence staining is widely used in the subcellular localization study of interested proteins. During the study of the functions of chloroplast division proteins, immunofluorescence staining was frequently adopted. Previously, a method has been developed to study the localization of a chloroplast division protein, FtsZ. However, it is laborious and time-consuming. In this study, we report a modified immunofluorescence staining method, in which protoplasts were isolated from leaf tissues, and then fixed for immunofluorescence staining. The time of the experiment was significantly reduced to several hours. Furthermore, we used correction pen in the fixation procedure and a new way to coat the slide, which greatly saved the cost of the experiment. With the chloroplast division protein ARC6 as an example, we can get a good fluorescence signal. Moreover, the localization of ARC6 in two chloroplast division mutants, arc3 and arc5, and three other plant species, such as cabbage, radish and pea, was also successfully analyzed with our new method. Overall, the immunofluorescence staining method we reported here is very practical, and it significantly facilitates the visualization of the subcellular localization of interested proteins in plant cells.
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