ArticlePlant cell reports2016
A novel arrangement of zinc finger nuclease system for in vivo targeted genome engineering: the tomato LEC1-LIKE4 gene case.
Article in Plant cell reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 65 citations in OpenAlex.
- Comparative Transcriptome Analysis in Tomato Fruit Reveals Genes, Pathways, and Processes Affected by the LEC1-LIKE4 Transcription Factor.International journal of molecular sciences · 2025Article
- Genome Editing of thePlants (Basel, Switzerland) · 2025Article
- Multi-Omics Approaches Against Abiotic and Biotic Stress-A Review.Plants (Basel, Switzerland) · 2025Review
- Advancing vegetable genetics with gene editing: a pathway to food security and nutritional resilience in climate-shifted environments.Functional & integrative genomics · 2025Review
- Recent advances in site-specific transgene insertion into the maize genome using recombinases and genome editing endonucleases.Frontiers in plant science · 2025Review
- Recent Advances in Tomato Gene Editing.International journal of molecular sciences · 2024Review
- Improvement of crop production in controlled environment agriculture through breeding.Frontiers in plant science · 2024Review
- Review of CRISPR/Cas Systems on Detection of Nucleotide Sequences.Foods (Basel, Switzerland) · 2023Review
- Genome editing for improving nutritional quality, post-harvest shelf life and stress tolerance of fruits, vegetables, and ornamentals.Frontiers in genome editing · 2023Review
- Potentials of genotypes, morpho-physio-biochemical traits, and growing media on shelf life and future prospects of gene editing in tomatoes.Frontiers in genome editing · 2023Article
- Genetically modified organisms: adapting regulatory frameworks for evolving genome editing technologies.Biological research · 2022Review
- Potential of Industrial Hemp for Phytoremediation of Heavy Metals.Plants (Basel, Switzerland) · 2022Review
- CRISPR/Cas12a-based biosensing platform for the on-site detection of single-base mutants in gene-edited rice.Frontiers in plant science · 2022Article
- Advances in application of genome editing in tomato and recent development of genome editing technology.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Review
- Targeted plant improvement through genome editing: from laboratory to field.Plant cell reports · 2021Review
- Application of Genome Editing in Tomato Breeding: Mechanisms, Advances, and Prospects.International journal of molecular sciences · 2021Review
- Mutagenesis Approaches and Their Role in Crop Improvement.Plants (Basel, Switzerland) · 2019Article
- Mutation Breeding in Tomato: Advances, Applicability and Challenges.Plants (Basel, Switzerland) · 2019Review
- Genome editing for horticultural crop improvement.Horticulture research · 2019Review
- Genome Editing as a Tool for Fruit Ripening Manipulation.Frontiers in plant science · 2018Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageA selection-free, highly efficient targeted mutagenesis approach based on a novel ZFN monomer arrangement for genome engineering in tomato reveals plant trait modifications. How to achieve precise gene targeting in plants and especially in crops remains a long-sought goal for elucidating gene function and advancing molecular breeding. To address this issue, zinc finger nuclease (ZFN)-based technology was developed for the Solanum lycopersicum seed system. A ZFN architecture design with an intronic sequence between the two DNA recognition sites was evaluated for its efficiency in targeted gene mutagenesis. Custom engineered ZFNs for the developmental regulator LEAFY-COTYLEDON1-LIKE4 (L1L4) coding for the β subunit of nuclear factor Y, when transiently expressed in tomato seeds, cleaved the target site and stimulated imperfect repair driven by nonhomologous end-joining, thus, introducing mutations into the endogenous target site. The successful in planta application of the ZFN platform resulted in L1L4 mutations which conferred heterochronic phenotypes during development. Our results revealed that sequence changes upstream of the DNA binding domain of L1L4 can lead to phenotypic diversity including fruit organ. These results underscore the utility of engineered ZFN approach in targeted mutagenesis of tomato plant which may accelerate translational research and tomato breeding.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.