ArticleBMC microbiology2016
Characterization of Staphylococcus aureus isolates from raw milk sources in Victoria, Australia.
Article in BMC microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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37 citing papers in PubMed, 88 citations in OpenAlex.
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- Whole genome sequencing identifies exotoxin and antimicrobial resistance profiles of Staphylococcus aureus from Maine dairy farms.BMC veterinary research · 2025Article
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- Expression of mastitis causing fibrinogen binding protein of gram positive bacteria in genetically engineered switchgrass and antibodies production in mice.Physiology and molecular biology of plants : an international journal of functional plant biology · 2024Article
- Effect of silver nanoparticles and REP-PCR typing of Staphylococcus aureus isolated from various sources.Scientific reports · 2024Article
- Rapid detection of major enterotoxin genes and antibiotic resistance of Staphylococcus aureus isolated from raw milk in the Yazd province, Iran.Veterinary medicine and science · 2024Article
- Investigation of Microbial Quality of Milk and Milk Products and Isolations of Some Major Bacteria in the Central and Northwestern Zones of Tigray, Ethiopia.Veterinary medicine international · 2024Article
- Isolation and identification ofHeliyon · 2023Article
- Occurrence and Characteristics ofMolecules (Basel, Switzerland) · 2022Article
- Usefulness of molecular typing methods for epidemiological and evolutionary studies ofSaudi journal of biological sciences · 2022Review
- Persistence of Staphylococcus spp. in milk from cows undergoing homeopathy to control subclinical mastitis.BMC veterinary research · 2022Article
- Shared antibiotic resistance and virulence genes in Staphylococcus aureus from diverse animal hosts.Scientific reports · 2022Article
- Article
- Isolation and Identification ofVeterinary medicine international · 2022Article
- Comparative genomics study ofFrontiers in microbiology · 2022Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHighly pathogenic strains of Staphylococcus aureus can cause disease in both humans and animals. In animal species, including ruminants, S. aureus may cause severe or sub-clinical mastitis. Dairy animals with mastitis frequently shed S. aureus into the milk supply which can lead to food poisoning in humans. The aim of this study was to use genotypic and immunological methods to characterize S. aureus isolates from milk-related samples collected from 7 dairy farms across Victoria.
resultsA total of 30 S. aureus isolates were collected from milk and milk filter samples from 3 bovine, 3 caprine and 1 ovine dairy farms across Victoria, Australia. Pulsed Field Gel Electrophoresis (PFGE) identified 11 distinct pulsotypes among isolates; all caprine and ovine isolates shared greater than 80 % similarity regardless of source. Conversely, bovine isolates showed higher diversity. Multi-Locus Sequence Typing (MLST) identified 5 different sequence types (STs) among bovine isolates, associated with human or ruminant lineages. All caprine and ovine isolates were ST133, or a single allele variant of ST133. Two new novel STs were identified among isolates in this study (ST3183 and ST3184). With the exception of these 2 new STs, eBURST analysis predicted all other STs to be founding members of their associated clonal complexes (CCs). Analysis of genetic markers revealed a diverse range of classical staphylococcal enterotoxins (SE) among isolates, with 11 different SEs identified among bovine isolates, compared with just 2 among caprine and ovine isolates. None of the isolates contained mecA, or were resistant to oxacillin. The only antibiotic resistance identified was that of a single isolate resistant to penicillin; this isolate also contained the penicillin resistance gene blaZ. Production of SE was observed at 16 °C and/or 37 °C in milk, however no SE production was detected at 12 °C.
conclusionAlthough this study characterized a limited number of isolates, bovine-associated isolates showed higher genetic diversity than their caprine or ovine counterparts. This was also reflected in a more diverse SE repertoire among bovine isolates. Very little antibiotic resistance was identified among isolates in this study. These results suggest maintaining the milk cold chain will minimise any risk from SE production and highlights the need to prevent temperature abuse.
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